台湾儿童的SEN病毒感染：传播途径及其在输血和肝脏疾病中的作用。

SEN virus infection in children in Taiwan: transmission route and role in blood transfusion and liver diseases.

作者信息

Hsu Hong-Yuan, Ni Yen-Hsuan, Chiang Cheng Lun, Shyu Ming-Kwang, Chen Huey-Ling, Chang Mei-Hwei

机构信息

Department of Pediatrics, National Taiwan University College of Medicine and National Taiwan University Hospital, Taipei.

出版信息

Pediatr Infect Dis J. 2006 May;25(5):390-4. doi: 10.1097/01.inf.0000214962.04264.f2.

DOI:10.1097/01.inf.0000214962.04264.f2

PMID:16645500

Abstract

BACKGROUND

SEN virus (SENV) is a newly discovered DNA virus. We conducted this study to evaluate potential modes of SENV transmission and the pathogenic effect of SENV on liver diseases in children.

METHODS

Polymerase chain reaction was used to detect 2 SENV variant (SENV-D and SENV-H) DNA in sera from healthy individuals and diseased children. Nucleotide sequence of SENV was determined by direct sequencing.

RESULTS

SENV infection was assessed in healthy individuals, including 50 newborns (sera collected from the umbilical cord), 24 infants, 46 preschool children (aged 1-6 years), 42 school children of an age before that of the first sexual experience (aged 7-12 years), 62 adolescents (13-18 years), 72 young adults (19-30 years) and 32 adults (>30 years). The prevalence of SENV-D and/or SENV-H (SENV-D/H) viremia in each group was 0%, 17%, 24%, 24%, 27%, 33% and 40%, respectively. The prevalence of SENV-D/H viremia in 18 children with non-A to E hepatitis, 64 thalassemic children, 80 children transfused during cardiac surgery, 30 children with chronic hepatitis B, 9 children with chronic hepatitis C and 32 infants with biliary atresia was 11%, 61%, 80%, 83%, 67% and 50%, respectively. SENV was found more frequently in all patient groups than in 174 age-matched controls (P < 0.01), with the exception of non-A to E hepatitis (11% versus 24% in the control group; P = 0.27). In 2 infants with proven intrauterine hepatitis B viral infection, identical SENV-D nucleotide sequence existed in both the maternal and neonate serum. Elevated alanine aminotransferase concentrations were rarely observed in children who acquired isolated SENV viremia because of transfusion for surgery. Infection with SENV in children with chronic hepatitis C virus or hepatitis B viral infection was not associated with higher peak alanine aminotransferase values.

CONCLUSION

SENV is transmitted mainly via nonparenteral daily contact and frequently occurs early in life. Transfusion can significantly increase the rate of SENV viremia. SENV does not appear to cause hepatitis in children.

摘要

背景

SEN病毒（SENV）是一种新发现的DNA病毒。我们开展本研究以评估SENV的潜在传播方式以及SENV对儿童肝脏疾病的致病作用。

方法

采用聚合酶链反应检测健康个体和患病儿童血清中的2种SENV变异体（SENV-D和SENV-H）DNA。通过直接测序确定SENV的核苷酸序列。

结果

对健康个体进行了SENV感染评估，包括50名新生儿（采集脐带血血清）、24名婴儿、46名学龄前儿童（1 - 6岁）、42名首次性经历前年龄段的学龄儿童（7 - 12岁）、62名青少年（13 - 18岁）、72名青年成年人（19 - 30岁）和32名成年人（>30岁）。各年龄组中SENV-D和/或SENV-H（SENV-D/H）病毒血症的患病率分别为0%、17%、24%、24%、27%、33%和40%。18例非甲至戊型肝炎患儿、64例地中海贫血患儿、80例心脏手术期间输血的患儿、30例慢性乙型肝炎患儿、9例慢性丙型肝炎患儿和32例胆道闭锁婴儿中SENV-D/H病毒血症的患病率分别为11%、61%、80%、83%、67%和50%。除非甲至戊型肝炎外（11%对比对照组的24%；P = 0.27），所有患者组中SENV的检出频率均高于174名年龄匹配的对照组（P < 0.01）。在2例经证实宫内感染乙型肝炎病毒的婴儿中，母婴血清中存在相同的SENV-D核苷酸序列。因手术输血而单独感染SENV病毒血症的儿童中，很少观察到丙氨酸转氨酶浓度升高。慢性丙型肝炎病毒或乙型肝炎病毒感染患儿感染SENV与丙氨酸转氨酶峰值升高无关。