Runx3（Aml2）和Runx1（Aml1）的时空表达模式表明其在小鼠胚胎发育过程中具有非冗余功能。

Spatial and temporal expression pattern of Runx3 (Aml2) and Runx1 (Aml1) indicates non-redundant functions during mouse embryogenesis.

作者信息

Levanon D, Brenner O, Negreanu V, Bettoun D, Woolf E, Eilam R, Lotem J, Gat U, Otto F, Speck N, Groner Y

机构信息

Department of Molecular Genetics, The Weizmann Institute of Science, Rehovot 76100, Israel.

出版信息

Mech Dev. 2001 Dec;109(2):413-7. doi: 10.1016/s0925-4773(01)00537-8.

DOI:10.1016/s0925-4773(01)00537-8

PMID:11731260

Abstract

The human RUNX3/AML2 gene belongs to the 'runt domain' family of transcription factors that act as gene expression regulators in major developmental pathways. Here, we describe the expression pattern of Runx3 during mouse embryogenesis compared to the expression pattern of Runx1. E10.5 and E14.5-E16.5 embryos were analyzed using both immunohistochemistry and beta-galactosidase activity of targeted Runx3 and Runx1 loci. We found that Runx3 expression overlapped with that of Runx1 in the hematopoietic system, whereas in sensory ganglia, epidermal appendages, and developing skeletal elements, their expression was confined to different compartments. These data provide new insights into the function of Runx3 and Runx1 in organogenesis and support the possibility that cross-regulation between them plays a role in embryogenesis.

摘要

人类RUNX3/AML2基因属于转录因子的“ runt结构域”家族，在主要发育途径中作为基因表达调节因子发挥作用。在这里，我们描述了与Runx1的表达模式相比，Runx3在小鼠胚胎发生过程中的表达模式。使用针对Runx3和Runx1基因座的免疫组织化学和β-半乳糖苷酶活性分析了E10.5和E14.5 - E16.5胚胎。我们发现Runx3的表达与造血系统中Runx1的表达重叠，而在感觉神经节、表皮附属器和发育中的骨骼元件中，它们的表达局限于不同的区域。这些数据为Runx3和Runx1在器官发生中的功能提供了新的见解，并支持它们之间的交叉调节在胚胎发生中起作用的可能性。