作为用于细菌基因操作的可控基因靶向载体的II组内含子。
Group II introns as controllable gene targeting vectors for genetic manipulation of bacteria.
作者信息
Karberg M, Guo H, Zhong J, Coon R, Perutka J, Lambowitz A M
机构信息
Institute for Cellular and Molecular Biology, Department of Chemistry and Biochemistry, School of Biological Sciences, University of Texas at Austin, Austin, TX 78712, USA.
出版信息
Nat Biotechnol. 2001 Dec;19(12):1162-7. doi: 10.1038/nbt1201-1162.
Mobile group II introns can be retargeted to insert into virtually any desired DNA target. Here we show that retargeted group II introns can be used for highly specific chromosomal gene disruption in Escherichia coli and other bacteria at frequencies of 0.1-22%. Furthermore, the introns can be used to introduce targeted chromosomal breaks, which can be repaired by transformation with a homologous DNA fragment, enabling the introduction of point mutations. Because of their wide host range, mobile group II introns should be useful for genetic engineering and functional genomics in a wide variety of bacteria.
可移动的II组内含子能够被重新靶向，以插入到几乎任何所需的DNA靶点中。在此我们表明，重新靶向的II组内含子可用于在大肠杆菌和其他细菌中以0.1%-22%的频率进行高度特异性的染色体基因破坏。此外，这些内含子可用于引入靶向染色体断裂，其可通过用同源DNA片段转化来修复，从而实现点突变的引入。由于其广泛的宿主范围，可移动的II组内含子应可用于多种细菌的基因工程和功能基因组学研究。
Zotero 插件