Wang Lixuan, Hu Jing, Li Kun, Zhao Yuliang, Zhu Motao
CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, China, Beijing 100190, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
iScience. 2024 Aug 22;27(9):110791. doi: 10.1016/j.isci.2024.110791. eCollection 2024 Sep 20.
Probiotics typically refer to microorganisms that have been identified for their health benefits, and they are added to foods or supplements to promote the health of the host. A growing number of probiotic strains have been identified lately and developed into valuable regulatory pharmaceuticals for nutritional and medical applications. Gene editing technologies play a crucial role in addressing the need for safe and therapeutic probiotics in disease treatment. These technologies offer valuable assistance in comprehending the underlying mechanisms of probiotic bioactivity and in the development of advanced probiotics. This review aims to offer a comprehensive overview of gene editing technologies applied in the engineering of both traditional and next-generation probiotics. It further explores the potential for on-demand production of customized products derived from enhanced probiotics, with a particular emphasis on the future of gene editing in the development of live biotherapeutics.
益生菌通常是指那些已被确认对健康有益的微生物，它们被添加到食品或补充剂中以促进宿主健康。最近已鉴定出越来越多的益生菌菌株，并将其开发成用于营养和医学应用的有价值的调节药物。基因编辑技术在满足疾病治疗中对安全且具治疗作用的益生菌的需求方面发挥着关键作用。这些技术在理解益生菌生物活性的潜在机制以及先进益生菌的开发方面提供了宝贵的帮助。本综述旨在全面概述应用于传统和下一代益生菌工程的基因编辑技术。它进一步探讨了从增强型益生菌按需生产定制产品的潜力，特别强调了基因编辑在活体生物治疗剂开发中的未来。
