Holdaway-Clarke T L, Walker N A, Reid R J, Hepler P K, Overall R L
School of Biological Sciences A12, University of Sydney, Sydney, NSW 2006, Australia.
Protoplasma. 2001;215(1-4):184-90. doi: 10.1007/BF01280313.
The effect of lowering cytoplasmic pH on the ionic conductivity of higher-plant plasmodesmata was investigated with corn (Zea mays L. cv. Black Mexican Sweet) suspension culture cells. Exposure to butyric acid decreased the cytoplasmic pH by 0.8 units. Intercellular communication was monitored by electrophysiological techniques that allowed the measurement of membrane resistances of sister cells and the electrical resistance of the plasmodesmata connecting them. The decrease in cytoplasmic pH did not affect the resistance of plasmodesmata, despite the fact that the butyric acid treatment more than doubled the concentration of cytoplasmic calcium. This is discussed in light of previous findings that increases in cytoplasmic calcium increase the electrical resistance of plasmodesmata.
利用玉米(Zea mays L. cv. Black Mexican Sweet)悬浮培养细胞研究了降低细胞质pH值对高等植物胞间连丝离子电导率的影响。用丁酸处理使细胞质pH值降低了0.8个单位。通过电生理技术监测细胞间通讯,该技术能够测量姐妹细胞的膜电阻以及连接它们的胞间连丝的电阻。尽管丁酸处理使细胞质钙浓度增加了一倍多,但细胞质pH值的降低并未影响胞间连丝的电阻。根据之前细胞质钙增加会增加胞间连丝电阻的研究结果对这一现象进行了讨论。