Suppr超能文献

马铃薯纺锤块茎类病毒RNA复制子的遗传变异性

Genetic variability of potato spindle tuber viroid RNA replicon.

作者信息

Góra-Sochacka A, Candresse T, Zagórski W

机构信息

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa.

出版信息

Acta Biochim Pol. 2001;48(2):467-76.

Abstract

The genetic continuity of the potato spindle tuber viroid (PSTVd) genome was analysed after infection of tomato plants with cloned cDNAs of parental strains. During the six weeks of the experiment, several new sequence variants appeared. The sequence variants detected in the progeny population induced sequence-specific disease symptoms. The PSTVd genome therefore follows the pattern expected for typical pseudo-strains propagating in plants as a population of similar sequences. Assessing further the replicon continuity, a PSTVd cDNA mutant with a deletion in the central conserved region was constructed and proven to be non-infectious. Surprisingly, in a sub-population of potato transformants expressing the same deleted PSTVd RNA an infectious viroid was detected. This suggests specific transcript conversion followed by recovery of the full-length pathogen genome.

摘要

用亲本菌株的克隆cDNA感染番茄植株后,分析了马铃薯纺锤块茎类病毒(PSTVd)基因组的遗传连续性。在实验的六周内,出现了几个新的序列变体。在子代群体中检测到的序列变体引发了序列特异性的病害症状。因此,PSTVd基因组遵循了典型假病毒株在植物中作为相似序列群体传播时预期的模式。为进一步评估复制子的连续性,构建了一个在中央保守区域有缺失的PSTVd cDNA突变体,并证明其无感染性。令人惊讶的是,在表达相同缺失PSTVd RNA的马铃薯转化体亚群体中检测到了一种有感染性的类病毒。这表明发生了特定的转录物转化,随后全长病原体基因组得以恢复。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验