番茄中 CRISPR-Cas13a 基因编辑系统对马铃薯纺锤块茎类病毒感染的抗性及.
Resistance of the CRISPR-Cas13a Gene-Editing System to Potato Spindle Tuber Viroid Infection in Tomato and .
机构信息
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
National Citrus Engineering Research Center, Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Citrus Research Institute, Southwest University, Chongqing 400712, China.
出版信息
Viruses. 2024 Aug 31;16(9):1401. doi: 10.3390/v16091401.
Gene-editing technology, specifically the CRISPR-Cas13a system, has shown promise in breeding plants resistant to RNA viruses. This system targets RNA and, theoretically, can also combat RNA-based viroids. To test this, the CRISPR-Cas13a system was introduced into tomato plants via transient expression and into through transgenic methods, using CRISPR RNAs (crRNAs) targeting the conserved regions of both sense and antisense genomes of potato spindle tuber viroid (PSTVd). In tomato plants, the expression of CRISPR-Cas13a and crRNAs substantially reduced PSTVd accumulation and alleviated disease symptoms. In transgenic plants, the PSTVd levels were lower as compared to wild-type plants. Several effective crRNAs targeting the PSTVd genomic RNA were also identified. These results demonstrate that the CRISPR-Cas13a system can effectively target and combat viroid RNAs, despite their compact structures.
基因编辑技术，特别是 CRISPR-Cas13a 系统，在培育抗 RNA 病毒的植物方面显示出了潜力。该系统靶向 RNA，理论上也可以对抗基于 RNA 的类病毒。为了验证这一点，通过瞬时表达将 CRISPR-Cas13a 系统引入番茄植物，并通过转基因方法将其引入 ，使用靶向马铃薯纺锤形块茎类病毒（PSTVd）正、反义基因组保守区的 CRISPR 核糖核酸（crRNA）。在番茄植物中，CRISPR-Cas13a 和 crRNA 的表达显著降低了 PSTVd 的积累并缓解了疾病症状。与野生型植物相比，转基因 植物中的 PSTVd 水平较低。还鉴定了几个针对 PSTVd 基因组 RNA 的有效 crRNA。这些结果表明，CRISPR-Cas13a 系统可以有效地靶向和对抗类病毒 RNA，尽管它们的结构紧凑。
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