血清中苯双甲吗啉的葡萄糖氧化酶法测定

GLC determination of phendimetrazine in serum.

作者信息

Hundt H K, Clark E C, Müller F O

出版信息

J Pharm Sci. 1975 Jun;64(6):1041-3. doi: 10.1002/jps.2600640638.

Abstract

A sensitive, specific, and quantitative GLC method for the determination of phendimetrazine in serum is described. The procedure involves the addition of an internal standard to serum samples, followed by extraction at pH 13 into toluene. The extracted bases are back-extracted into 1 ml of 4 M hydrochloric acid and again into 100 mu1 of chloroform after making the 4 M hydrochloric acid extract basic with 1.5 ml of 4 M sodium hydroxide. The sensitivity of the method is such that 25 ng of material can be detected in 5 ml of serum.

摘要

本文描述了一种灵敏、特异且定量的气相色谱法，用于测定血清中的苯双甲吗啉。该方法包括向血清样品中加入内标物，然后在pH 13条件下用甲苯萃取。萃取得到的碱用1 ml 4 M盐酸反萃取，再加入1.5 ml 4 M氢氧化钠使4 M盐酸萃取液呈碱性后，再次用100 μl氯仿萃取。该方法的灵敏度使得在5 ml血清中可检测到25 ng的物质。