Lawrence Carolyn J, Malmberg Russell L, Muszynski Michael G, Dawe R Kelly
University of Georgia, Department of Botany, Athens, GA 30602, USA.
J Mol Evol. 2002 Jan;54(1):42-53. doi: 10.1007/s00239-001-0016-y.
We have reconstructed the evolution of the anciently derived kinesin superfamily using various alignment and tree-building methods. In addition to classifying previously described kinesins from protists, fungi, and animals, we analyzed a variety of kinesin sequences from the plant kingdom including 12 from Zea mays and 29 from Arabidopsis thaliana. Also included in our data set were four sequences from the anciently diverged amitochondriate protist Giardia lamblia. The overall topology of the best tree we found is more likely than previously reported topologies and allows us to make the following new observations: (1) kinesins involved in chromosome movement including MCAK, chromokinesin, and CENP-E may be descended from a single ancestor; (2) kinesins that form complex oligomers are limited to a monophyletic group of families; (3) kinesins that crosslink antiparallel microtubules at the spindle midzone including BIMC, MKLP, and CENP-E are closely related; (4) Drosophila NOD and human KID group with other characterized chromokinesins; and (5) Saccharomyces SMY1 groups with kinesin-I sequences, forming a family of kinesins capable of class V myosin interactions. In addition, we found that one monophyletic clade composed exclusively of sequences with a C-terminal motor domain contains all known minus end-directed kinesins.
我们使用了各种比对和建树方法，重构了古老起源的驱动蛋白超家族的进化历程。除了对先前已描述的来自原生生物、真菌和动物的驱动蛋白进行分类外，我们还分析了植物界的多种驱动蛋白序列，包括来自玉米的12种和来自拟南芥的29种。我们的数据集中还包括来自古老分化的无线粒体原生生物贾第虫的4种序列。我们发现的最优树的整体拓扑结构比先前报道的拓扑结构更具可信度，并且使我们能够得出以下新的观察结果：（1）参与染色体运动的驱动蛋白，包括MCAK、染色体驱动蛋白和CENP - E，可能起源于同一个祖先；（2）形成复杂寡聚体的驱动蛋白仅限于一个单系家族群；（3）在纺锤体中区交联反平行微管的驱动蛋白，包括BIMC、MKLP和CENP - E，亲缘关系密切；（4）果蝇NOD和人类KID与其他已鉴定的染色体驱动蛋白归为一组；（5）酿酒酵母SMY1与驱动蛋白 - I序列归为一组，形成一个能够与V类肌球蛋白相互作用的驱动蛋白家族。此外，我们发现一个仅由具有C末端运动结构域的序列组成的单系分支包含了所有已知的负端定向驱动蛋白。
