Goodson H V, Kang S J, Endow S A
Department of Biochemistry, Stanford University Medical School, CA 94305.
J Cell Sci. 1994 Jul;107 ( Pt 7):1875-84. doi: 10.1242/jcs.107.7.1875.
The rapidly expanding kinesin family of microtubule motor proteins includes proteins that are involved in diverse microtubule-based functions in the cell. Phylogenetic analysis of the motor regions of the kinesin proteins reveals at least five clearly defined groups that are likely to identify kinesins with different roles in basic cellular processes. Two of the groups are consistent with overall sequence similarity, while two groups contain proteins that are related in overall structure or function but show no significant sequence similarity outside the motor domain. One of these groups consists only of kinesin proteins with predicted C-terminal motor domains; another includes only kinesins required for mitotic spindle bipolarity. Drosophila Nod, presently an ungrouped protein, may represent a class of kinesins that, like the myosin I proteins, function as monomers. The analysis indicates that many types of kinesin proteins exist in eukaryotic organisms. At least two of the five groups identified in this analysis are expected to be present in most, or all, eukaryotes.
微管运动蛋白中迅速扩张的驱动蛋白家族包括参与细胞内多种基于微管功能的蛋白质。对驱动蛋白的运动区域进行系统发育分析，揭示了至少五个明确界定的组，这些组可能识别出在基本细胞过程中具有不同作用的驱动蛋白。其中两组与整体序列相似性一致，而另外两组包含在整体结构或功能上相关但在运动结构域之外没有显著序列相似性的蛋白质。其中一组仅由具有预测的C末端运动结构域的驱动蛋白组成；另一组仅包括有丝分裂纺锤体双极性所需的驱动蛋白。果蝇Nod目前是一种未分组的蛋白质，可能代表一类驱动蛋白，就像肌球蛋白I蛋白一样，作为单体发挥作用。该分析表明，真核生物中存在多种类型的驱动蛋白。预计在本次分析中确定的五组中至少有两组存在于大多数或所有真核生物中。
