Tsuchida T, Kato T, Yamaga M, Ikebe K, Oniki Y, Irie H, Takagi K
Department of Orthopaedic Surgery, Kumamoto University School of Medicine, Kumamoto 860-8556, Japan.
J Surg Res. 2001 Dec;101(2):238-41. doi: 10.1006/jsre.2001.6278.
Despite the established preservation method for major organs of perfusion followed by immersion at hypothermia, a standard preservation technique for skeletal muscle is still a matter of controversy. The purpose of this study is to examine the effect of perfusion on the preservation of skeletal muscle in amputated limbs.
The rat hindlimb was amputated for perfusion with Euro-Collins (EC) or University of Wisconsin (UW) solution at different perfusion pressures (40 or 100 cm-gravity). After certain ischemic periods (4 or 5 h), the skeletal muscle viability was determined by measuring the tissue content of adenosine triphosphate (ATP).
The UW solution perfusion group maintained better ATP levels than the EC solution group when the ischemic period was extended to 5 h. The perfusion pressure of 100 cm-gravity was more effective for preserving muscle viability than 40 cm-gravity with both EC and UW solutions.
UW solution might be adequate to preserve muscle viability and perfusion pressure is recommended at 100 cm-gravity rather than at minimal pressure (40 cm-gravity), which washes out stagnant blood.
尽管已确立了对主要器官先进行灌注然后低温浸泡的保存方法，但骨骼肌的标准保存技术仍存在争议。本研究的目的是检验灌注对截肢肢体骨骼肌保存的影响。
将大鼠后肢截肢，分别用欧林柯林斯（EC）溶液或威斯康星大学（UW）溶液在不同灌注压力（40或100厘米水柱）下进行灌注。在特定缺血期（4或5小时）后，通过测量三磷酸腺苷（ATP）的组织含量来确定骨骼肌活力。
当缺血期延长至5小时时，UW溶液灌注组比EC溶液组维持了更好的ATP水平。对于EC溶液和UW溶液，100厘米水柱的灌注压力在保存肌肉活力方面比40厘米水柱更有效。
UW溶液可能足以保存肌肉活力，建议灌注压力为100厘米水柱而非最小压力（40厘米水柱），后者可冲洗出停滞的血液。
