产气荚膜梭菌中pCW3编码的四环素抗性诱导涉及一个宿主编码因子。

Induction of pCW3-encoded tetracycline resistance in Clostridium perfringens involves a host-encoded factor.

作者信息

Johanesen P A, Lyras D, Rood J I

机构信息

Bacterial Pathogenesis Research Group, Monash University, Victoria 3800, Australia.

出版信息

Plasmid. 2001 Nov;46(3):229-32. doi: 10.1006/plas.2001.1543.

DOI:10.1006/plas.2001.1543

PMID:11735372

Abstract

The tetracycline resistance determinant Tet P, which is encoded by the conjugative plasmid pCW3 from Clostridium perfringens, is induced by subinhibitory concentrations of tetracycline. In this study we have shown that the inducible phenotype is strain dependent. When pCW3 is present in derivatives of the wild-type strains CW234 and CW362 resistance is inducible. However, transfer to derivatives of strain 13 leads to a constitutive phenotype that is only observed in this strain background. Based on these results it is proposed that induction of the pCW3-encoded tet(P) genes in C. perfringens requires a host-encoded factor that is either absent or nonfunctional in strain 13 derivatives.

摘要

由产气荚膜梭菌的接合质粒pCW3编码的四环素抗性决定簇Tet P，可被亚抑制浓度的四环素诱导。在本研究中，我们已表明可诱导表型是菌株依赖性的。当pCW3存在于野生型菌株CW234和CW362的衍生物中时，抗性是可诱导的。然而，转移至菌株13的衍生物会导致组成型表型，且仅在该菌株背景中观察到。基于这些结果，有人提出产气荚膜梭菌中pCW3编码的tet(P)基因的诱导需要一种宿主编码因子，该因子在菌株13的衍生物中不存在或无功能。