Hsu B M, Huang C
Department of Environmental Engineering and Health, Yuanpei Institute of Science and Technology, 306 Yuanpei Street, Hsinchu, Taiwan, Republic of China.
Biotechnol Prog. 2001 Nov-Dec;17(6):1114-8. doi: 10.1021/bp010111h.
Immunomagnetic separation (IMS) has been specified as a standard method for the measurement of Cryptosporidium in some countries. In this study, the IMS method was evaluated on the basis of the recovery efficiencies of Cryptosporidium oocysts at various IMS operation conditions. The average recovery for different Cryptosporidium concentrations in deionized water was 82.6 +/- 18.2% (n = 52). No significant change in recovery was observed by altering the debris ratio of the water samples. The efficiency was increased by prolonging the reaction time, and by increasing the amount of immunomagnetic beads. The recoveries of oocysts seeded in an Eppendorf with a small reaction volume were similar to those seeded in glass tubes with 10 times the reaction volume. The recovery efficiency of oocysts was reduced significantly when the reaction buffer was replaced by PBS. In conclusion, this method has good reproducibility and high recovery.
免疫磁珠分离法（IMS）在一些国家已被指定为测量隐孢子虫的标准方法。在本研究中，基于隐孢子虫卵囊在各种IMS操作条件下的回收效率对IMS方法进行了评估。去离子水中不同隐孢子虫浓度的平均回收率为82.6±18.2%（n = 52）。改变水样的杂质比例时，未观察到回收率有显著变化。通过延长反应时间和增加免疫磁珠的量，效率得以提高。接种于反应体积小的艾本德管中的卵囊回收率与接种于反应体积为其10倍的玻璃管中的相似。当反应缓冲液被PBS替代时，卵囊的回收效率显著降低。总之，该方法具有良好的重现性和高回收率。
