Smith A M, Morris T, Justin T, Michaeli D, Watson S A
The Academic Unit of Cancer Studies, University Hospital, Nottingham, UK.
Aliment Pharmacol Ther. 2001 Dec;15(12):1981-8. doi: 10.1046/j.1365-2036.2001.01081.x.
Gastrimmune is an immunogenic form of gastrin. It raises in situ antibodies against two proliferative forms of gastrin: amidated and glycine-extended gastrin-17. It has been shown to have a therapeutic action in several in vivo tumour models. Following immunization, due to the complex equilibrium that exists between the antibodies and gastrin, it is not technically feasible to assay for free gastrin.
To determine the effect of Gastrimmune-induced antigastrin antibodies on acid secretion.
A rat gastric fistula model was used. Animals (six per group) were immunized with a control immunogen or ascending doses of Gastrimmune. Acid output was measured following infusion of increasing doses of gastrin-17 and pentagastrin.
Gastrimmune-induced antibodies significantly reduced gastrin-17-stimulated acid output compared to control animals (Gastrimmune at 200 microg/rat vs. control; acid output following 30 ng gastrin-17, 0.01 vs. 0.16, P < 0.001; following 120 ng gastrin-17, 0.022 vs. 0.29, P < 0.001).
Gastrimmune significantly inhibits gastrin-17-stimulated acid output. This biological assay suggests that the antigastrin antibodies effectively bind gastrin-17. In addition to its use as an antineoplastic agent, Gastrimmune may have a role as an acid-decreasing agent in oesophagogastric pathology.
胃免疫素是胃泌素的一种免疫原性形式。它能原位产生针对两种增殖形式胃泌素的抗体：酰胺化胃泌素-17和甘氨酸延伸型胃泌素-17。在多种体内肿瘤模型中已证实其具有治疗作用。免疫后，由于抗体与胃泌素之间存在复杂的平衡关系，检测游离胃泌素在技术上不可行。
确定胃免疫素诱导的抗胃泌素抗体对胃酸分泌的影响。
采用大鼠胃瘘模型。动物（每组6只）用对照免疫原或递增剂量的胃免疫素进行免疫。在输注递增剂量的胃泌素-17和五肽胃泌素后测量胃酸分泌量。
与对照动物相比，胃免疫素诱导的抗体显著降低了胃泌素-17刺激的胃酸分泌量（200μg/大鼠的胃免疫素与对照相比；30ng胃泌素-17后的胃酸分泌量，0.01对0.16，P<0.001；120ng胃泌素-17后的胃酸分泌量，0.022对0.29，P<0.001）。
胃免疫素显著抑制胃泌素-17刺激的胃酸分泌量。该生物学检测表明抗胃泌素抗体能有效结合胃泌素-17。除用作抗肿瘤药物外，胃免疫素在食管胃病理学中可能还具有作为胃酸减少剂的作用。
