赤霉素对番茄（Solanum lycopersicum）中两个新的花特异性基因表达的调控。

Regulation of expression of two novel flower-specific genes from tomato (Solanum lycopersicum) by gibberellin.

作者信息

Van den Heuvel Koen J, Van Lipzig Rosalinde H, Barendse Gerard W, Wullems George J

机构信息

Department of Experimental Botany, Research Group Molecular Plant Physiology, University of Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands.

出版信息

J Exp Bot. 2002 Jan;53(366):51-9. doi: 10.1093/jexbot/53.366.51.

DOI:10.1093/jexbot/53.366.51

PMID:11741041

Abstract

Two flower-specific cDNAs have been isolated after differential screening of an anther cDNA library. This library was constructed 48 h after GA(3) treatment of buds of the GA-deficient gib-1 mutant of tomato. Northern blot analysis during flower development in tomato demonstrated that the expression of both genes is regulated by gibberellins (GAs). Application of GA(3) to developmentally arrested gib-1 flower buds induced new expression of tgas100 mRNA 48 h post-treatment, while an increased accumulation of tgas105 mRNA was found after 8 h. In situ analyses showed the spatial distribution of the expression of both genes within the tomato flower. One of the deduced polypeptides (TGAS105) displays similarities to cysteine-rich extensin-like proteins, while the other (TGAS100) shows significant homology with a stamen-specific gene of Antirrhinum majus. Based on the deduced protein sequences, the possible function of the encoded proteins is discussed.

摘要

通过对花药cDNA文库进行差异筛选，分离出了两个花特异性cDNA。该文库是在对番茄GA缺陷型gib-1突变体的芽进行GA(3)处理48小时后构建的。番茄花发育过程中的Northern印迹分析表明，这两个基因的表达均受赤霉素（GAs）调控。对发育停滞的gib-1花芽施用GA(3)后，处理48小时后诱导tgas100 mRNA出现新的表达，而在8小时后发现tgas105 mRNA的积累增加。原位分析显示了这两个基因在番茄花中的表达空间分布。推导的一种多肽（TGAS105）与富含半胱氨酸的类伸展蛋白相似，而另一种（TGAS100）与金鱼草的一个雄蕊特异性基因具有显著同源性。基于推导的蛋白质序列，讨论了编码蛋白的可能功能。