Sibéril Yann, Thiersault Martine, Nepumoceno Gonçalo, Doireau Pierre, Gantet Pascal
EA 2106, Biomolécules et Biotechnologies Végétales, Université de Tours, UFR des Sciences et Techniques, Laboratoire de Physiologie Végétale, Parc de Grandmont, 37200 Tours, France.
J Exp Bot. 2002 Jan;53(366):149-50.
A novel cDNA (Crubie2) encoding ubiquitin-conjugating enzyme E2 was isolated from a Catharanthus roseus cDNA library. Sequence comparison with Arabidopsis thaliana E2 sequences revealed that CrUBIE2 is a member of a new plant E2 sub-family. Expression of Crubie2 is repressed in developing organs and down-regulated by cytokinin suggesting that a decrease in the ubiquitin-dependent proteolytic pathway may take part in the regulation of alkaloid biosynthesis in C. roseus cell suspensions.
从长春花cDNA文库中分离出一个编码泛素结合酶E2的新cDNA（Crubie2）。与拟南芥E2序列的序列比较表明，CrUBIE2是植物E2新亚家族的成员。Crubie2在发育中的器官中表达受到抑制，并受细胞分裂素下调，这表明泛素依赖性蛋白水解途径的减少可能参与长春花细胞悬浮液中生物碱生物合成的调控。
