Weninger Wolfgang Johann, Mohun Timothy
Integrative Morphology Group, Department of Anatomy, University of Vienna, Waehringerstrasse 13 A-1090 Wien, Austria.
Nat Genet. 2002 Jan;30(1):59-65. doi: 10.1038/ng785. Epub 2001 Dec 17.
We describe a technique suitable for routine three-dimensional (3-D) analysis of mouse embryos that is based on episcopic fluorescence images captured during serial sectioning of wax-embedded specimens. We have used this procedure to describe the cardiac phenotype and associated blood vessels of trisomic 16 (Ts16) and Cited2-null mutant mice, as well as the expression pattern of an Myf5 enhancer/beta-galactosidase transgene. The consistency of the images and their precise alignment are ideally suited for 3-D analysis using video animations, virtual resectioning or commercial 3-D reconstruction software packages. Episcopic fluorescence image capturing (EFIC) provides a simple and powerful tool for analyzing embryo and organ morphology in normal and transgenic embryos.
我们描述了一种适用于对小鼠胚胎进行常规三维（3-D）分析的技术，该技术基于在石蜡包埋标本连续切片过程中捕获的落射荧光图像。我们已使用此方法来描述16三体（Ts16）和Cited2基因敲除突变小鼠的心脏表型及相关血管，以及Myf5增强子/β-半乳糖苷酶转基因的表达模式。这些图像的一致性及其精确对齐非常适合使用视频动画、虚拟重切片或商业3-D重建软件包进行三维分析。落射荧光图像捕获（EFIC）为分析正常和转基因胚胎中的胚胎及器官形态提供了一个简单而强大的工具。
