Baratta J, Ha D H, Yu J, Robertson R T
Department of Anatomy and Neurobiology, College of Medicine, University of California-Irvine, Irvine, CA 92697-1280, USA.
Brain Res Dev Brain Res. 2001 Dec 14;132(1):15-21. doi: 10.1016/s0165-3806(01)00290-5.
Possible target preferences of basal forebrain cholinergic neurons were studied in organotypic slice cultures. Cholinergic neurons in slices of medial septum or substantia innominata send axons into both hippocampus and neocortex when co-cultured together. However, septal cholinergic axons course through adjacent slices of neocortex to reach and branch densely in slices of hippocampus, but septal axons seldom grow beyond adjacent hippocampal tissue to reach neocortex. In contrast, cholinergic axons from substantia innominata commonly grow through hippocampus to reach neocortex, and also grow through neocortex to reach hippocampus, with similar branching densities in each target. The greater density of septal axonal branches in hippocampus than in neocortex suggests a preference of septal axons for the hippocampal target.
在器官型脑片培养中研究了基底前脑胆碱能神经元可能的靶偏好。内侧隔区或无名质切片中的胆碱能神经元在共同培养时，其轴突会同时延伸至海马体和新皮层。然而，隔区胆碱能轴突穿过相邻的新皮层脑片，到达海马体脑片并在其中密集分支，但隔区轴突很少延伸至相邻海马组织之外以到达新皮层。相比之下，来自无名质的胆碱能轴突通常穿过海马体到达新皮层，也会穿过新皮层到达海马体，在每个靶区的分支密度相似。海马体中隔区轴突分支的密度大于新皮层，这表明隔区轴突对海马体靶区具有偏好。
