AMPA诱导新生大鼠脑内少突胶质细胞基因表达的抑制
AMPA-Induced suppression of oligodendroglial gene expression in neonatal rat brain.
作者信息
Xu H, Barks J D, Liu Y Q, Silverstein F S
机构信息
Department of Pediatrics, University of Michigan, Room 8301 MSRB3, Box 0646, 1150 West Medical Center Drive, Ann Arbor, MI 48109-0646, USA.
出版信息
Brain Res Dev Brain Res. 2001 Dec 31;132(2):175-8. doi: 10.1016/s0165-3806(01)00306-6.
Oligodendroglia are susceptible to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-mediated excitotoxicity. We hypothesized that analysis of oligodendroglial gene expression would provide a sensitive indicator of acute excitotoxic oligodendroglial injury in the immature brain. AMPA (2 nmol) was injected intracerebroventricularly in 7-day-old rats. Proteolipid protein and myelin basic protein encoding mRNAs were evaluated 24-48 h later by semi-quantitative reverse transcription-polymerase chain reaction assays. AMPA markedly suppressed brain tissue content of oligodendroglial mRNAs.
少突胶质细胞易受α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）介导的兴奋性毒性作用影响。我们推测，对少突胶质细胞基因表达的分析将为未成熟大脑中急性兴奋性毒性少突胶质细胞损伤提供一个敏感指标。将AMPA（2 nmol）脑室内注射到7日龄大鼠体内。24 - 48小时后，通过半定量逆转录-聚合酶链反应分析评估编码髓鞘脂蛋白和髓鞘碱性蛋白的mRNA。AMPA显著抑制少突胶质细胞mRNA的脑组织含量。
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