从皮下脂肪活检中分离出的大、小阻力动脉的功能异质性：对血管病理生理学研究的启示。

Functional heterogeneity of large and small resistance arteries isolated from biopsies of subcutaneous fat: implications for investigation of vascular pathophysiology.

作者信息

Hadoke P W, McIntyre C A, Gray G A, Buckley C H

机构信息

Department of Medical Sciences, Western General Hospital, Hugh Robson Building, University of Edinburgh, Crewe Road, Edinburgh EH4 2XU, Scotland, UK.

出版信息

Gen Pharmacol. 2000 Sep;35(3):119-27. doi: 10.1016/s0306-3623(01)00085-4.

DOI:10.1016/s0306-3623(01)00085-4

PMID:11744233

Abstract

Few studies using human subcutaneous resistance arteries acknowledge the possibility of functional heterogeneity in these vessels. Large ( approximately 500 microm) and small (> or = 200 microm) resistance arteries (n=11) and veins (n=5) were identified using physical, structural and functional criteria in 14 biopsies of human gluteal fat. Endothelium-dependent relaxation was not evident in veins, while, unlike small resistance arteries (E(max) 95.74+/-1.86%; -log IC(50) 7.28+/-0.09), large resistance arteries with an intact endothelium failed to respond to acetylcholine. These results suggest that large resistance arteries may lack muscarinic receptors on the endothelium and emphasise the importance of careful vessel selection and characterisation in studies using human resistance arteries.

摘要

很少有使用人体皮下阻力动脉的研究认识到这些血管中功能异质性的可能性。在14份人体臀肌脂肪活检样本中，通过物理、结构和功能标准识别出大（约500微米）、小（≥200微米）阻力动脉（n = 11）和静脉（n = 5）。静脉中内皮依赖性舒张不明显，而与小阻力动脉不同（最大舒张率E(max) 95.74±1.86%；-log半数抑制浓度IC(50) 7.28±0.09），具有完整内皮的大阻力动脉对乙酰胆碱无反应。这些结果表明，大阻力动脉的内皮可能缺乏毒蕈碱受体，并强调了在使用人体阻力动脉的研究中仔细选择和表征血管的重要性。