Wilson J A, Pearce J D, Ayazi Shamlou P
The Advanced Centre for Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK.
Biotechnol Bioeng. 2001 Dec 20;75(6):733-40. doi: 10.1002/bit.10045.
This study assesses the suitability of sieving as a scaleable technique for the separation of adult nematodes from infective juveniles, the latter is an effective bioinsecticide whereas the former is waste material resulting from the fermentation process. Batch and semibatch experiments using conventional flow-assisted wet sieving and a novel cross-flow sieving technique were used to study the separation of juveniles from adult nematodes. The experiments were carried out using small-scale devices and the data were analyzed in terms of the screen effectiveness factor. The results were used to identify the sieve size and operating conditions for optimum juvenile recovery. It was found that, for a given species of nematode, optimum recovery was achieved when sieving was carried out in the cross-flow mode, the maximum recovery being a function of the size of the screen. Industrial-scale self-cleaning equipment capable of large-scale continuous screening was used to confirm the capacity of the small-scale operation for scale-up. Experimental results with this unit showed that in continuous operation sieving time is an additional parameter that influences separation performance.
本研究评估了筛分作为一种可扩展技术用于从感染性幼虫中分离成年线虫的适用性，后者是一种有效的生物杀虫剂，而前者是发酵过程产生的废料。使用传统的流动辅助湿筛法和一种新型错流筛分技术进行了分批和半分批实验，以研究从成年线虫中分离幼虫的情况。实验使用小规模装置进行，并根据筛网效率因子对数据进行分析。结果用于确定最佳幼虫回收率的筛网尺寸和操作条件。研究发现，对于给定种类的线虫，在错流模式下进行筛分可实现最佳回收率，最大回收率是筛网尺寸的函数。使用能够进行大规模连续筛分的工业规模自清洁设备来确认小规模操作放大的能力。该装置的实验结果表明，在连续操作中，筛分时间是影响分离性能的另一个参数。
