Marushige Y, Marushige K
Biochim Biophys Acta. 1975 Sep 22;403(1):180-91. doi: 10.1016/0005-2744(75)90020-0.
When isolated bull sperm chromatin is incubated with 0.1 M 2-mercaptoethanol at pH 8, an extensive proteolytic degradation of sperm histone occurs, being accompanied by a marked swelling of the chromatin masses. The degradation of sperm histone is strongly inhibited by monovalent or divalent metal ions. The protease found in isolated bull sperm chromatin possesses properties indistinguishable from those of an acrosomal protease of trypsin-type, acrosin (EC 3.4.21.10), and requires a combination of NaCl, urea and 2-mercaptoethanol for its extraction. Evidence suggests that the protease travels along chromatin strands and hydrolyzes essentially all the sperm histone molecules within the chromatin masses.
当将分离的公牛精子染色质在pH 8的条件下与0.1 M 2-巯基乙醇一起孵育时，精子组蛋白会发生广泛的蛋白水解降解，同时染色质团块会明显肿胀。精子组蛋白的降解受到一价或二价金属离子的强烈抑制。在分离的公牛精子染色质中发现的蛋白酶具有与胰蛋白酶型顶体蛋白酶（顶体素，EC 3.4.21.10）无法区分的特性，并且其提取需要氯化钠、尿素和2-巯基乙醇的组合。有证据表明，该蛋白酶沿着染色质链移动并水解染色质团块内基本上所有的精子组蛋白分子。
