Humpe A, Riggert J, Koch S, Legler T J, Munzel U, Köhler M
Department of Transfusion Medicine, Georg-August-University, Göttingen, Germany.
J Clin Apher. 2001;16(3):109-13. doi: 10.1002/jca.1020.
Some data exist on the influence of leukapheresis volume on the number of harvested peripheral blood hematopoietic progenitor cells (HPC), but less is known about the influence on the composition of HPC. We therefore performed a prospective, randomized crossover trial to evaluate the effect of large-volume (LVL) vs. normal-volume leukapheresis (NVL) on subpopulations of CD34(+) cells in the harvest product of 15 patients with breast cancer and 8 patients with non-Hodgkin's lymphoma. Patients were randomly assigned to start either with an LVL on day 1 followed by an NVL on day 2 or vice versa. The number of HPC, the extraction efficiency defined as difference between yield in the harvest and decrease in peripheral blood, and the relative proportion as well as the absolute numbers of CD34(+) cells coexpressing CD38, CD90, HLA-DR, CD117, CD7, CD19, CD41, or CD33 were evaluated. There was no significant difference with regard to the percentages of the subsets on comparison of LVL to NVL procedures. Only the absolute median number of CD34(+)HLA-DR(-) cells was significantly (P=0.02) higher in LVL harvests compared with the corresponding NVL components, which can be explained on the basis of the higher yield and the higher extraction efficiency in LVL compared with NVL. LVL results in a higher yield of CD34(+) cells and leads to an intra-apheresis recruitment of HPC but the relative composition of the harvested CD34(+) cells is not changed significantly. In addition, the amount of early, HLA-DR(-), hematopoietic HPC seems to be increased by an LVL.
关于白细胞单采量对采集的外周血造血祖细胞（HPC）数量的影响已有一些数据，但对HPC组成的影响了解较少。因此，我们进行了一项前瞻性随机交叉试验，以评估大容量（LVL）与正常容量白细胞单采（NVL）对15例乳腺癌患者和8例非霍奇金淋巴瘤患者采集产物中CD34(+)细胞亚群的影响。患者被随机分配，要么在第1天开始进行LVL，然后在第2天进行NVL，要么反之。评估了HPC的数量、定义为采集产量与外周血减少量之差的提取效率，以及共表达CD38、CD90、HLA-DR、CD117、CD7、CD19、CD41或CD33的CD34(+)细胞的相对比例和绝对数量。在比较LVL和NVL程序时，各亚群的百分比没有显著差异。与相应的NVL成分相比，仅LVL采集中CD34(+)HLA-DR(-)细胞的绝对中位数数量显著更高（P = 0.02），这可以基于LVL与NVL相比更高的产量和更高的提取效率来解释。LVL导致CD34(+)细胞产量更高，并导致采集过程中HPC的募集，但采集的CD34(+)细胞的相对组成没有显著变化。此外，大容量白细胞单采似乎会增加早期造血HPC（HLA-DR(-)）的数量。
