Frederiksen H, Sjuve R, Arner A, Uvelius B
Department of Urology, Lund University Hospital, Lund, Sweden.
Neurourol Urodyn. 2001;20(6):685-97. doi: 10.1002/nau.1020.
The aim of the present study was to determine to what extent adult rats can produce new contracting bladder muscle and to see if such newly formed bladder tissue possesses characteristic mechanical properties or whether the ability to recover mechanically is so pronounced that the prehistory of the bladder is unimportant. Subtotal cystectomy was performed in adult female rats, leading to a pronounced decrease in total bladder weight. At 10 weeks, bladder weight had normalized. The histological appearance of such bladders was similar to that of the controls. Active and passive length-tension relations for the detrusor muscle were determined in controls and up to 10 weeks after surgery. Immediately after surgery active and passive forces showed a leftward shift and maximum active force decreased markedly. With time the length-tension curves shifted back to normal, but a decreased active force still remained at 10 weeks. Detrusor actin concentration and detrusor myosin/actin ratio were unaffected by the subtotal cystectomy. Intermediate filament protein/actin ratio showed a significant but transitory increase. We conclude that there is a remarkable recovery of detrusor muscle function after subtotal cystectomy, leading to a normalization of optimum length for active force and a net synthesis of contractile and cytoskeletal proteins. The ability to produce active force does, however, not fully recover.
本研究的目的是确定成年大鼠能够在多大程度上产生新的收缩性膀胱肌肉，并观察这种新形成的膀胱组织是否具有特征性的力学特性，或者其机械恢复能力是否如此显著以至于膀胱的既往史并不重要。对成年雌性大鼠进行了膀胱部分切除术，导致膀胱总重量显著下降。10周时，膀胱重量恢复正常。此类膀胱的组织学外观与对照组相似。在对照组以及术后长达10周的时间里，测定了逼尿肌的主动和被动长度-张力关系。术后即刻，主动和被动力均向左移位，最大主动力显著下降。随着时间推移，长度-张力曲线恢复正常，但在10周时主动力仍有所下降。膀胱部分切除术未影响逼尿肌肌动蛋白浓度以及逼尿肌肌球蛋白/肌动蛋白比率。中间丝蛋白/肌动蛋白比率有显著但短暂的升高。我们得出结论，膀胱部分切除术后逼尿肌功能有显著恢复，导致主动力最佳长度恢复正常，以及收缩蛋白和细胞骨架蛋白的净合成。然而，产生主动力的能力并未完全恢复。
