Takanami Iwao, Takeuchi Ken, Watanabe Hideomi, Yanagawa Takashi, Takagishi Kenji
First Department of Surgery, Teikyo University School of Medicine, Itabashi-ku, Tokyo 173, Japan.
Oncol Rep. 2002 Jan-Feb;9(1):125-8.
The cell motility of tumor cells is an integral component of tumor invasion and metastasis. Autocrine motility factor (AMF) stimulates cell motility via a receptor (R)-mediated signal pathway. We studied the correlation between level of AMF-R gene expression by reverse transcription-polymerase chain reaction and cell motility by phagokinetic track assay with or without AMF in lung cancer cell lines. Lung cancer cell lines with higher AMF-R gene expression tended to have greater cell motility than those with lower AMF-R gene expression with or without AMF. Most of lung cancer cell lines showed significantly increased motility with the stimulation of AMF. We conclude that the presence of higher AMF-R gene expression and tumor cell motility via receptor in response to the stimulation of AMF could be an important aspect in the invasion and metastasis of lung cancer cell lines.
肿瘤细胞的细胞运动性是肿瘤侵袭和转移的一个重要组成部分。自分泌运动因子（AMF）通过受体（R）介导的信号通路刺激细胞运动。我们通过逆转录聚合酶链反应研究了肺癌细胞系中AMF-R基因表达水平与采用或不采用AMF的吞噬动力学轨迹试验检测的细胞运动性之间的相关性。无论有无AMF，AMF-R基因表达较高的肺癌细胞系往往比AMF-R基因表达较低的肺癌细胞系具有更强的细胞运动性。大多数肺癌细胞系在AMF刺激下运动性显著增强。我们得出结论，较高的AMF-R基因表达以及肿瘤细胞通过受体对AMF刺激产生的运动性可能是肺癌细胞系侵袭和转移的一个重要方面。
