Latuszyńska J, Luty S, Raszewski G, Tokarska-Rodak M, Przebirowska D, Przylepa E, Haratym-Maj A
Department of Pathomorphology, Institute of Agricultural Medicine, P.O.Box 185, 20-950 Lublin, Poland.
Ann Agric Environ Med. 2001;8(2):163-70.
The aim of the study was to evaluate the neurotoxic effect of a dermally-applied mixture of chlorpyrifos and cypermethrin in rats based on cognitive function, activity of the blood cholinesterase and brain acetylcholinesterase, as well as histologic brain examination. Nurelle D 550 EC (500 g of chlorpyrifos and 50 g of cypermethrin) was used in the study. The application liquid was in the form of a water solution. The investigation covered eight groups of animals: six experimental groups and two control groups, of 15 rats each. Experimental groups received 5.6 mg/cm2 chlorpyrifos and 0.5 mg/cm2 cypermethrin, or 27.8 mg/cm2 chlorpyrifos and 2.7 mg/cm2 cypermethrin dermally, for one day, one week and four weeks, except for Saturdays and Sundays. The preparations examined were applied to the tail skin of rats. The animals were anaesthetized at the end of exposure period. Plasma cholinesterase and brain acetylcholinesterase activities were determined. The brain for histological examination was perfused with a solution of methanol, formalin and glacial acetic acid, and the sections stained by the Nissel method. The behaviour of the animals was evaluated in the open field test four times: before exposure, and after one, two and four weeks of the experiment. The results of the study showed that chlorpyrifos and cypermethrin applied in a mixture caused an inhibition of cholinesterase and acetylcholinesterase activity and elicited the pycnosis of brain neurocytes.
本研究的目的是基于认知功能、血液胆碱酯酶和脑乙酰胆碱酯酶的活性以及脑组织学检查，评估经皮肤涂抹毒死蜱和氯氰菊酯混合物对大鼠的神经毒性作用。本研究使用了Nurelle D 550 EC（500克毒死蜱和50克氯氰菊酯）。涂抹液为水溶液形式。研究涵盖八组动物：六个实验组和两个对照组，每组15只大鼠。实验组大鼠除周六和周日外，连续一天、一周和四周经皮肤接受5.6毫克/平方厘米毒死蜱和0.5毫克/平方厘米氯氰菊酯，或27.8毫克/平方厘米毒死蜱和2.7毫克/平方厘米氯氰菊酯。受试制剂涂抹于大鼠尾部皮肤。在暴露期结束时将动物麻醉。测定血浆胆碱酯酶和脑乙酰胆碱酯酶活性。用于组织学检查的大脑用甲醇、福尔马林和冰醋酸溶液灌注，切片采用尼氏法染色。在旷场试验中对动物的行为进行四次评估：暴露前、实验进行一周、两周和四周后。研究结果表明，毒死蜱和氯氰菊酯混合使用会导致胆碱酯酶和乙酰胆碱酯酶活性受到抑制，并引发脑神经元细胞固缩。
