Pizarro J C, Vulliez-le Normand B, Riottot M M, Budkowska A, Bentley G A
Unité d' Immunologie Structurale, Institut Pasteur, Paris, France.
FEBS Lett. 2001 Dec 14;509(3):463-8. doi: 10.1016/s0014-5793(01)03190-8.
The monoclonal antibody 5a19, raised against the ay serotype of hepatitis B virus, binds to the segment of the preS1 region comprising residues 37-43, which is implicated in attachment of the virus to hepatocytes. The dissociation constant, derived from kinetic studies using surface plasmon resonance techniques, is in the low nanomolar range. The nucleotide sequence of the variable domains has been determined and the corresponding germ-line genes have been identified. The three-dimensional structure of the Fab fragment has been determined by X-ray crystallography to 2.6 A resolution.
针对乙肝病毒ay血清型产生的单克隆抗体5a19，与前S1区包含37 - 43位氨基酸残基的片段结合，该片段与病毒附着于肝细胞有关。通过表面等离子体共振技术进行动力学研究得出的解离常数处于低纳摩尔范围。已确定可变区的核苷酸序列，并鉴定出相应的种系基因。Fab片段的三维结构已通过X射线晶体学确定，分辨率为2.6埃。
