Huang Chin-Cheng, Lee Fan, Tu Wen-Jeng, Lee Shu-Hwae, Huang Ten-Shiang, Lin Yeou-Liang, Jong Ming-Hwa, Lin Shih-Yuh
Department of Hog Cholera, National Institute for Animal Health, Council of Agriculture, 376 Chung-Cheng Road, Tamsui, Taipei 251, Taiwan, ROC.
Vet Microbiol. 2002 Feb 4;84(4):317-26. doi: 10.1016/s0378-1135(01)00471-0.
The O/Taiwan/99 foot-and-mouth disease virus (FMDV), a South Asian topotype of serotype O, was introduced into Taiwan in 1999. The Chinese yellow cattle infected by the virus did not develop clinical lesions under experimental and field conditions. A blocking enzyme-linked immunosorbent assay (ELISA) kit with the 3AB antigen, a polypeptide of FMDV non-structural (NS) proteins, was used to evaluate the development and duration of anti-3AB antibodies, proving active viral replication, in the Chinese yellow cattle. The specificity of the assay was 99%, as was established with negative sera from regularly vaccinated and from naïve cattle. The sensitivity tested with sera from naturally infected animals was approximately 64% and it was lower than that obtained by serum neutralization (SN) test. Under experimental infection, the Chinese yellow cattle developed lower anti-3AB antibodies than that developed in other species. Duration of anti-3AB antibodies was traced in two herds of naturally infected animals, indicating that anti-3AB antibodies persisted for approximately 6 months after outbreaks. On the basis of this study, we propose that the Chinese yellow cattle may have natural resistance, which limits viral replication and reduces the development of anti-3AB antibodies.
O/台湾/99口蹄疫病毒（FMDV）是O型血清型的一种南亚拓扑型，于1999年传入台湾。在实验和田间条件下，感染该病毒的中国黄牛未出现临床病变。使用一种以FMDV非结构（NS）蛋白的多肽3AB抗原为基础的阻断酶联免疫吸附测定（ELISA）试剂盒，来评估中国黄牛体内抗3AB抗体的产生情况和持续时间，以证明病毒的活跃复制。该检测方法的特异性为99%，这是通过常规接种疫苗的牛和未接种疫苗的牛的阴性血清确定的。用自然感染动物的血清进行检测，其灵敏度约为64%，低于血清中和（SN）试验的灵敏度。在实验感染情况下，中国黄牛产生的抗3AB抗体低于其他物种。在两群自然感染动物中追踪了抗3AB抗体的持续时间，表明疫情爆发后抗3AB抗体持续约6个月。基于这项研究，我们提出中国黄牛可能具有天然抗性，这限制了病毒复制并减少了抗3AB抗体的产生。
