Jackson E L, Willis N, Mercer K, Bronson R T, Crowley D, Montoya R, Jacks T, Tuveson D A
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Genes Dev. 2001 Dec 15;15(24):3243-8. doi: 10.1101/gad.943001.
Adenocarcinoma of the lung is the most common form of lung cancer, but the cell of origin and the stages of progression of this tumor type are not well understood. We have developed a new model of lung adenocarcinoma in mice harboring a conditionally activatable allele of oncogenic K-ras. Here we show that the use of a recombinant adenovirus expressing Cre recombinase (AdenoCre) to induce K-ras G12D expression in the lungs of mice allows control of the timing and multiplicity of tumor initiation. Through the ability to synchronize tumor initiation in these mice, we have been able to characterize the stages of tumor progression. Of particular significance, this system has led to the identification of a new cell type contributing to the development of pulmonary adenocarcinoma.
肺腺癌是肺癌最常见的形式，但这种肿瘤类型的起源细胞和进展阶段尚未完全清楚。我们构建了一种携带致癌性K-ras条件性激活等位基因的小鼠肺腺癌新模型。在此我们表明，利用表达Cre重组酶的重组腺病毒（腺病毒Cre）在小鼠肺中诱导K-ras G12D表达，能够控制肿瘤起始的时间和多发性。通过同步这些小鼠肿瘤起始的能力，我们得以描绘肿瘤进展的阶段。特别重要的是，该系统已导致鉴定出一种对肺腺癌发展有贡献的新细胞类型。
