RapA是SWI2/SNF2的细菌同源物，可促进转录过程中的RNA聚合酶循环利用。
RapA, a bacterial homolog of SWI2/SNF2, stimulates RNA polymerase recycling in transcription.
作者信息
Sukhodolets M V, Cabrera J E, Zhi H, Jin D J
机构信息
Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
出版信息
Genes Dev. 2001 Dec 15;15(24):3330-41. doi: 10.1101/gad.936701.
Abstract
We report that RapA, an Escherichia coli RNA polymerase (RNAP)-associated homolog of SWI2/SNF2, is capable of dramatic activation of RNA synthesis. The RapA-mediated transcriptional activation in vitro depends on supercoiled DNA and high salt concentrations, a condition that is likely to render the DNA superhelix tightly compacted. Moreover, RapA activates transcription by stimulating RNAP recycling. Mutational analyses indicate that the ATPase activity of RapA is essential for its function as a transcriptional activator, and a rapA null mutant exhibits a growth defect on nutrient plates containing high salt concentrations in vivo. Thus, RapA acts as a general transcription factor and an integral component of the transcription machinery. The mode of action of RapA in remodeling posttranscription or posttermination complexes is discussed.
摘要
我们报道，RapA是一种与大肠杆菌RNA聚合酶（RNAP）相关的SWI2/SNF2同源物，能够显著激活RNA合成。RapA在体外介导的转录激活依赖于超螺旋DNA和高盐浓度，这种条件可能会使DNA超螺旋紧密压缩。此外，RapA通过刺激RNAP循环来激活转录。突变分析表明，RapA的ATPase活性对于其作为转录激活剂的功能至关重要，并且rapA缺失突变体在体内含有高盐浓度的营养平板上表现出生长缺陷。因此，RapA作为一种通用转录因子和转录机制的一个组成部分。本文还讨论了RapA在重塑转录后或终止后复合物中的作用模式。
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