Kalmanzon E, Aknin-Herrman R, Rahamim Y, Carmeli S, Barenholz Y, Zlotkin E
Institute of Life Sciences, The Hebrew University, 91904 Jerusalem, Israel.
Cell Mol Biol Lett. 2001;6(4):971-84.
Pahutoxin a quaternary ammonium salt surfactant serves as an active ingredient in the defensive skin secretion of various marine trunkfish (Ostracociidae). In the defensive skin secretion of the Red Sea trunkfish, Ostracion cubicus the effect of PHN is amplified due to the existence of non toxic polypeptides which act as (a) PHN - chelators and (b) potentiators. The secretion of the Red Sea trunkfish includes an additional category of pharmacologically active polypeptides represented by boxin [7] which similarly to PHN they independently kill fish exclusively through medium application. By the aid of radiolabeled PHN and a fish gill membrane preparation a series of equilibrium saturation binding assays were carry out which demonstrate that PHN performs its biological defensive function via receptors and not due to its surface activity. The gill membranes of the trunkfish were shown to be devoid of PHNreceptors. The pharmacological, ecological and environmental implications of the above data are discussed.
帕胡毒素是一种季铵盐表面活性剂，是各种海洋箱鲀（箱鲀科）皮肤防御分泌物中的活性成分。在红海箱鲀（立方箱鲀）的皮肤防御分泌物中，由于存在无毒多肽，帕胡毒素的作用得到增强，这些无毒多肽起到（a）帕胡毒素螯合剂和（b）增效剂的作用。红海箱鲀的分泌物还包括另一类具有药理活性的多肽，以博辛[7]为代表，与帕胡毒素类似，它们仅通过介质作用就能独立杀死鱼类。借助放射性标记的帕胡毒素和鱼鳃膜制剂，进行了一系列平衡饱和结合试验，结果表明帕胡毒素通过受体发挥其生物防御功能，而非因其表面活性。研究表明，箱鲀的鳃膜没有帕胡毒素受体。文中讨论了上述数据的药理学、生态学和环境意义。
