Yakabi Koji, Iwabuchi Hitoshi, Nakamura Takashi, Endo Keiko, Fukunaga Yoshie, Kumaki Iku, Takayama Kiyoshige
Third Department of Internal Medicine, Teikyo University School of Medicine, 3426-3 Anegasaki, Ichihara, Chiba 299-0111, Japan.
Neurosci Lett. 2002 Jan 11;317(2):57-60. doi: 10.1016/s0304-3940(01)02448-x.
The present study examined whether the central neurons are involved in the stimulatory action of gastrin on the secretion of gastric acid. Gastrin (20 microg), which was examined and ascertained to induce a marked increase in gastric acid secretion in gastric-lumen perfused rats, was intravenously injected in Wistar rats under anesthesia with pentobarbital sodium. In the experiments, 1 h after injecting gastrin, rats were perfused and fixed, the brain was removed and sectioned at 40 microm thickness. Every fourth section was treated with anti-c-Fos antiserum, and c-Fos protein was immunohistochemically stained using the avidin-biotin complex method. It was found that c-Fos protein was expressed in neurons of the lateral habenular nucleus, the central nucleus amygdala, the lateral parabrachial nucleus in the pons, and the complex area of the nucleus of the solitary tract and the dorsal motor nucleus of the vagus nerve in the medulla oblongata. The control rats were injected with saline solution, and the brain sections were processed similarly as described above. c-Fos protein was expressed in few neurons in the nuclei above in the control rats. These results suggest that gastrin released into the circulation might stimulate central neurons which, in turn, may relate to the control mechanism for the secretion of gastric acid.
本研究检测了中枢神经元是否参与胃泌素对胃酸分泌的刺激作用。在戊巴比妥钠麻醉下，给Wistar大鼠静脉注射经检测并确定能使胃腔灌注大鼠胃酸分泌显著增加的胃泌素（20微克）。实验中，注射胃泌素1小时后，对大鼠进行灌注和固定，取出大脑并切成40微米厚的切片。每隔4张切片用抗c-Fos抗血清处理，采用抗生物素蛋白-生物素复合物法对c-Fos蛋白进行免疫组织化学染色。结果发现，c-Fos蛋白在外侧缰核、杏仁中央核、脑桥外侧臂旁核以及延髓孤束核与迷走神经背运动核的复合区域的神经元中表达。给对照大鼠注射生理盐水，脑切片的处理方法与上述相同。在对照大鼠中，上述核团中只有少数神经元表达c-Fos蛋白。这些结果表明，释放到循环中的胃泌素可能刺激中枢神经元，而中枢神经元可能与胃酸分泌的控制机制有关。
