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哈维贝内克氏菌中NADH特异性和NADPH特异性FMN还原酶的鉴定。

Identification of NADH-specific and NADPH-specific FMN reductases in Beneckea harveyi.

作者信息

Gerlo E, Charlier J

出版信息

Eur J Biochem. 1975 Sep 15;57(2):461-7. doi: 10.1111/j.1432-1033.1975.tb02321.x.

Abstract

Distinct FMN reductases specific for NADH and NADPH were identified in extracts of Beneckea harveyi. These enzymes differ in their physical (molecular weight, thermostability) as well as in their chemical properties (binding constants for NADH and NADPH). The NADH-specific enzyme is more efficient than the NADPH-specific one with respect to the bioluminescent reaction.

摘要

在哈维氏贝内克氏菌提取物中鉴定出了分别对NADH和NADPH具有特异性的不同黄素单核苷酸还原酶。这些酶在物理性质(分子量、热稳定性)以及化学性质(对NADH和NADPH的结合常数)方面存在差异。就生物发光反应而言,NADH特异性酶比NADPH特异性酶更高效。

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