Kong W, Wang D
Department of Pathophysiology, Tongji Medical College, Science and Technology University of Huazhong, Wuhan 430030, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2001 Sep;24(9):531-3.
To elucidate the effect of chronic hypoxia on the cyclooxygenase-2 mRNA expression in pulmonary artery smooth muscle cells of rats.
The gene expressions of COX-1 and COX-2 during acute hypoxia were measured with semi-quantified RT-PCR in the 2nd, 4th and 6th subculture of intrapulmonary artery smooth muscle cells (PASMC) of wistar rats under normoxic and continuous hypoxic condition separately.
In all three generations, the expressions of COX-1 had no significant changes in hypoxia, while the COX-2 mRNA expression was higher in chronic hypoxic group than that in normoxic group and elevated with the duration of hypoxia exposure. After exposure to acute hypoxia, the augmentation of COX-2 mRNA expression in PASMC was higher in chronic hypoxic group than in normoxic group in all three subcultures.
Chronic hypoxia could enhance the mRNA expression of cyclooxygenase-2 caused by acute hypoxia in PASMC of rats. It might be responsible for the lower HPV under chronic hypoxia.
阐明慢性低氧对大鼠肺动脉平滑肌细胞中环氧化酶-2 mRNA表达的影响。
分别在常氧和持续低氧条件下，用半定量逆转录-聚合酶链反应（RT-PCR）检测第2代、第4代和第6代Wistar大鼠肺内动脉平滑肌细胞（PASMC）在急性低氧过程中COX-1和COX-2的基因表达。
在所有三代细胞中，低氧时COX-1的表达无显著变化，而慢性低氧组COX-2 mRNA表达高于常氧组，且随低氧暴露时间延长而升高。在所有三代细胞中，急性低氧暴露后，慢性低氧组PASMC中COX-2 mRNA表达的增加高于常氧组。
慢性低氧可增强大鼠PASMC中急性低氧所致的环氧化酶-2 mRNA表达。这可能是慢性低氧时肺血管低反应性降低的原因。
