[血小板对单核细胞促凝血活性的影响]
[Effect of platelet on procoagulant activity of monocyte].
作者信息
Xu J, He X, Xiong S
机构信息
Hemostatic Physiology Laboratory, Xiangya Medical College, Central Southern University, Changsha 410078, China.
出版信息
Zhonghua Xue Ye Xue Za Zhi. 2001 Sep;22(9):478-80.
PMID:11758230
Abstract
OBJECTIVE
To investigate the effect of platelet on procoagulant activity of mononcyte(M).
METHODS
The procoagulant activity of M lysates was measured by one stage clotting time assay, the tissue factor (TF) activity and the role of P-selectin were confirmed by specific monoclonal antibodies (McAb).
RESULTS
- Activated M challenged by lipopolysaccharide(LPS) had augmented procoagulant activity and this effect was blocked by TF McAb. 2. Procoagulant activity of M and activated M increased significantly with the presence of activated platelets. 3. The enhancement effect of activated platelet on the procoagulant activity of M was blocked by P-selectin McAb.
CONCLUSION
TF expression of M contributes to the procoagulant activity of Ms and activated platelet enhances the procoagulant activity via P-selectin.
摘要
目的
研究血小板对单核细胞（M）促凝活性的影响。
方法
采用一期凝血时间测定法检测M裂解物的促凝活性，用特异性单克隆抗体（McAb）证实组织因子（TF）活性及P-选择素的作用。
结果
- 脂多糖（LPS）刺激激活的M促凝活性增强，且此效应被TF McAb阻断。2. 活化血小板存在时，M及活化M的促凝活性显著增加。3. 活化血小板对M促凝活性的增强作用被P-选择素McAb阻断。
结论
M的TF表达有助于M的促凝活性，活化血小板通过P-选择素增强促凝活性。
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