人源单克隆抗心磷脂抗体对血小板功能及单核细胞组织因子表达的影响。
Effects of human monoclonal anticardiolipin antibodies on platelet function and on tissue factor expression on monocytes.
作者信息
Reverter J C, Tàssies D, Font J, Khamashta M A, Ichikawa K, Cervera R, Escolar G, Hughes G R, Ingelmo M, Ordinas A
机构信息
Hospital Clínic, Barcelona, Spain.
出版信息
Arthritis Rheum. 1998 Aug;41(8):1420-7. doi: 10.1002/1529-0131(199808)41:8<1420::AID-ART11>3.0.CO;2-U.
OBJECTIVE
To investigate the effect of human monoclonal anticardiolipin antibodies (aCL) on platelet interaction with the subendothelium under flow conditions and on tissue factor (TF) expression on normal monocytes.
METHODS
Three monoclonal IgM aCL (TM1B3, GR1D5, and EY2C9) and 2 affinity-purified IgM aCL were studied. Immunoglobulins were added to normal blood and perfused through chambers containing denuded vascular segments. Platelet interactions were morphometrically evaluated by determining the percentage of total surface covered by platelets (PCS) or by large aggregates of thrombi platelets (TP). Expression of TF on monocytes was measured after immunoglobulin incubation with normal mononuclear cells.
RESULTS
Significant increases in the total PCS and expression of TF were observed using all aCL. Increased levels of TP were induced by all aCL except EY2C9 (obtained from a patient without thrombosis). Previous incubations of these aCL with subendothelial surfaces did not increase platelet interaction.
CONCLUSION
The effects of aCL on platelet function may help to explain the pathophysiology of thrombosis in the antiphospholipid syndrome.
目的
研究人源单克隆抗心磷脂抗体（aCL）在流动条件下对血小板与内皮下层相互作用以及对正常单核细胞组织因子（TF）表达的影响。
方法
研究了三种单克隆IgM aCL（TM1B3、GR1D5和EY2C9）以及两种亲和纯化的IgM aCL。将免疫球蛋白加入正常血液中，并通过含有去内皮血管段的腔室进行灌注。通过测定血小板覆盖的总表面积百分比（PCS）或血栓性血小板大聚集体（TP）来形态计量评估血小板相互作用。在用免疫球蛋白孵育正常单核细胞后，测量单核细胞上TF的表达。
结果
使用所有aCL均观察到总PCS和TF表达显著增加。除EY2C9（来自无血栓形成患者）外，所有aCL均诱导TP水平升高。这些aCL与内皮下表面预先孵育并未增加血小板相互作用。
结论
aCL对血小板功能的影响可能有助于解释抗磷脂综合征中血栓形成的病理生理学。
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