Boubelík M, Lengerová A, Zelený V
Folia Biol (Praha). 1975;21(2):81-6.
Agglutinability by H-2 antisera of neonatal erythrocytes appears early in some mouse strains (such as C57BL/10) and late in others (A, CBA, etc). This might be due to interstrain differences of the postulated temporal genes controlling in this case the developmental expression of H-2 gene products A similarr effect might be simulated by interstrain variation in neonatal size of erythrocytes (possibly reflecting their stage of maturation from the generally larger blasts) which could indirectly affect agglutinability through a different density of H-2 sites on the cell surface. Such correlation was, however, not found in this study. A possible involvement of an extracellular control mechanism was also ruled out by demonstrating that in allogeneic radiation chimaeras created reciprocally from late and early strain mice (used as irradiated recipients and donors of curative cell inocula) the appearance of donor-specific H-2 agglutinability conforms to the donor's timing type being unaffected by the contrasting host's environment. The delayed agglutinability in a late strain was further shown to concern not only neonatal erythrocytes, but also those recruited from stem cells in "adult" bone marrow. Finally, two strains (C67BL/10 and CBA) classified as, respectively, early and late by H-2 agglutinability were typed similarly on the basis of the time course of 59Fe-incorporation rate/mg. spleen in newly formed syngeneic radiation chimaeras. The question whether this coincidence is due to chance or to H-2 linkage of a common or rather separate control mechanisms is discussed.
新生小鼠红细胞对H - 2抗血清的凝集性在某些小鼠品系（如C57BL/10）中出现得早，而在其他品系（A、CBA等）中出现得晚。这可能是由于假定的控制H - 2基因产物发育表达的时间基因存在品系间差异。类似的效应可能由新生红细胞大小的品系间差异模拟（这可能反映了它们从通常较大的母细胞开始的成熟阶段），而这可能通过细胞表面H - 2位点密度不同间接影响凝集性。然而，本研究未发现这种相关性。通过证明在由晚期和早期品系小鼠相互创建的同种异体辐射嵌合体（用作照射受体和治疗性细胞接种物的供体）中，供体特异性H - 2凝集性的出现符合供体的时间类型，不受宿主环境差异的影响，排除了细胞外控制机制的可能参与。晚期品系中凝集性延迟进一步表明不仅涉及新生红细胞，还涉及从“成年”骨髓干细胞募集的红细胞。最后，根据新形成的同基因辐射嵌合体中脾脏每毫克59Fe掺入率的时间进程，对分别被H - 2凝集性分类为早期和晚期的两个品系（C67BL/10和CBA）进行了类似的分型。讨论了这种巧合是由于偶然还是由于共同或相当独立的控制机制与H - 2连锁的问题。
