Nakamura A, Furuta H, Maeda H, Nagamatsu Y, Yoshimoto A
Tsukuba R&D Center, Fuji Oil Co., Ltd., Ibaraki, Japan.
Biosci Biotechnol Biochem. 2001 Oct;65(10):2249-58. doi: 10.1271/bbb.65.2249.
Soybean soluble polysaccharides (SSPS) extracted from soybean cotyledons have a pectin-like structure. The core polysaccharides after treatments with four kinds of hemicellulases and a pectinase contained approximately equal numbers of L-rhamnose and D-galacturonate residues, suggesting the presence of the rhamnogalacturonan (RG) I structure consisting of the diglycosyl repeating unit, -4)-alpha-D-GalpA-(1-->2)-alpha-L-Rhap-(1-. The lengths of RG chains were calculated as approximately 15, 28, and 100 diglycosyl repeats. The RG components linked to each other by intervention of galacturonan (GN) chains, constituting the backbone of SSPS. All arabinose residues, which constitute 21% of total SSPS sugars, were found to be in side chains from RG regions, and this was also true for galactose residues, which constitute 50% of total sugars. Of arabinose residues, 94% are present as alpha-1,3- or alpha-1,5-arabinans, and 89% of galactose residues were present as beta-1,4-galactans. Galactan chains are modified with arabinose, xylose, fucose, and glucose at the sites close to the RG regions.
从大豆子叶中提取的大豆可溶性多糖（SSPS）具有类似果胶的结构。用四种半纤维素酶和一种果胶酶处理后的核心多糖含有数量大致相等的L-鼠李糖和D-半乳糖醛酸残基，这表明存在由二糖基重复单元-4)-α-D-半乳糖醛酸-(1→2)-α-L-鼠李糖-(1-组成的鼠李半乳糖醛酸聚糖（RG）I结构。RG链的长度经计算约为15、28和100个二糖基重复单元。RG组分通过半乳糖醛酸聚糖（GN）链的介入相互连接，构成了SSPS的主链。构成SSPS总糖21%的所有阿拉伯糖残基均位于RG区域的侧链中，占总糖50%的半乳糖残基也是如此。在阿拉伯糖残基中，94%以α-1,3-或α-1,5-阿拉伯聚糖形式存在，89%的半乳糖残基以β-1,4-半乳聚糖形式存在。半乳聚糖链在靠近RG区域的位点被阿拉伯糖、木糖、岩藻糖和葡萄糖修饰。
