Weiss P A, Esterbauer H, Scherr F
Geburtshilfe Frauenheilkd. 1975 Jun;35(6):475-7.
A simplified method for the determination of urinary estriol in pregnancy by thin layer chromatography is described. After precipitation of the conjugated substances with ammonium sulfate a short hydrolysis is carried out. Estriol is extracted with diethylether and coupled with dark blue R salt to an azo-dye. After thin layer chromatographic isolation, a spectrophotometric determination from the chromatogram is done. The recovery rate is 94%. The mean error in serial determination is +/- 5,4%. 1 nanogram (10(-9) g) of estriol can be determined from the thin layer chromatogram. The time required to obtain a result is just over 1 hour.
本文描述了一种用薄层色谱法测定孕期尿雌三醇的简化方法。用硫酸铵沉淀结合物后进行短暂水解。用乙醚萃取雌三醇，并与深蓝色R盐偶联形成偶氮染料。薄层色谱分离后，对色谱图进行分光光度测定。回收率为94%。连续测定的平均误差为±5.4%。可从薄层色谱图中测定出1纳克（10⁻⁹克）的雌三醇。获得结果所需时间略超过1小时。
