Goodwin H S, Bicknese A R, Chien S N, Bogucki B D, Quinn C O, Wall D A
Departments of Pediatrics, Pediatric Research Institute, Cardinal Glennon Children's Hospital, Saint Louis University School of Medicine, St. Louis, Missouri, USA.
Biol Blood Marrow Transplant. 2001;7(11):581-8. doi: 10.1053/bbmt.2001.v7.pm11760145.
The stromal cell population in bone marrow has been the focus of much attention since it has been shown that this cell population can be expanded and differentiated into cells with the phenotype of bone, cartilage, muscle, stroma, neural, and fat cells. We evaluated umbilical cord blood (UCB) for the presence of these cells. From the mononuclear fraction of UCB, we demonstrated the presence of a subset of cells that have been maintained in continuous culture for more than 6 months (>10 passages). These adherent cell populations express adhesion molecules CD13+, CD29+, and CD44+, but not antigens of hematopoietic differentiation. Exposure of these cells to osteogenic agents resulted in an increase in expression of alkaline phosphatase and the appearance of hydroxyapatite nodules by Von Kossa staining. Incubation with adipogenic agents resulted in morphological change and staining with Oil Red O. In addition, when exposed to basic fibroblast growth factor and human epidermal growth factor the cells underwent changes consistent with cells of neural origin. These changes were demonstrated by a combination of immunofluorescent labeling and Western immunoblots for neural-specific markers. Thus, similar to what has been previously reported with bone marrow, cord blood contains a population of cells that can be expanded in culture and are able to express the phenotype of multiple lineages. Cord blood multilineage cells are slower to establish in culture, have a lower precursor frequency and a lower level of bone antigen expression, and lack constitutive expression of neural antigens when compared to bone marrow, suggesting a more primitive population. Cord blood may prove to be a new source of cells for cellular therapeutics for stromal, bone, and, potentially, neural repair.
骨髓中的基质细胞群体一直备受关注，因为已有研究表明，该细胞群体能够扩增并分化为具有骨、软骨、肌肉、基质、神经和脂肪细胞表型的细胞。我们评估了脐带血（UCB）中这些细胞的存在情况。从UCB的单核细胞部分，我们证实存在一个细胞亚群，该亚群已在连续培养中维持了6个月以上（>10代）。这些贴壁细胞群体表达黏附分子CD13 +、CD29 +和CD44 +，但不表达造血分化抗原。将这些细胞暴露于成骨因子会导致碱性磷酸酶表达增加，并通过冯·科萨染色出现羟基磷灰石结节。与成脂因子孵育会导致形态变化并用油红O染色。此外，当暴露于碱性成纤维细胞生长因子和人表皮生长因子时，这些细胞会发生与神经源性细胞一致的变化。这些变化通过免疫荧光标记和针对神经特异性标志物的蛋白质免疫印迹相结合得以证实。因此，与先前关于骨髓的报道相似，脐带血含有一群能够在培养中扩增并能够表达多种谱系表型的细胞。与骨髓相比，脐带血多谱系细胞在培养中建立较慢，前体频率较低，骨抗原表达水平较低，并且缺乏神经抗原的组成性表达，这表明其细胞群体更为原始。脐带血可能被证明是用于基质、骨以及潜在的神经修复的细胞治疗的新细胞来源。
