用于从亚洲蓍草中分离和鉴定极性愈创木烷型倍半萜内酯的色谱和光谱方法联用

Combination of chromatographic and spectroscopic methods for the isolation and characterization of polar guaianolides from Achillea asiatica.

作者信息

Glasl S, Gunbilig D, Narantuya S, Werner I, Jurenitsch J

机构信息

Institute of Pharmacognosy, University of Vienna, Centre of Pharmacy, Althanstrasse 14, A-1090 Vienna, Austria.

出版信息

J Chromatogr A. 2001 Nov 30;936(1-2):193-200. doi: 10.1016/s0021-9673(01)00947-5.

DOI:10.1016/s0021-9673(01)00947-5

PMID:11761000

Abstract

Four polar guaianolides, 8alpha-angeloxy-2alpha,4alpha, 10beta-trihydroxy-6betaH,7alphaH, 11betaH-1(5)-guaien- 12,6alpha-olide; 8alpha-angeloxy-1beta,2beta:4beta,5beta-diepoxy- 10beta-hydroxy-6betaH,7alphaH,11betaH-12,6alpha-guaianolide; 8alpha-angeloxy-4alpha, 10beta-dihydroxy-2-oxo-6betaH, 7alphaH, 11betaH- 1(5)-guaien- 12,6alpha-olide and 8-desacetyl-matricarin, were isolated from Achillea asiatica and characterized by TLC, MS, IR, HPLC and diode array detection. Purified extracts were separated by means of flash chromatography. HPLC separations were achieved using different methanol-water gradients as mobile phase and LiChrospher 100-RP8 5 microm or Zorbax SB-C8 3.5 microm as stationary phases. The chromatographical data are compared to those of the proazulene 8alpha-tigloxy-artabsin which shows antiinflammatory effects. By means of these characteristics the identification of the guaianolides with potential antiphlogistic properties is also possible from other sources.

摘要

从亚洲蓍草中分离出四种愈创木烷型愈创木内酯，分别为8α-当归酰氧基-2α,4α,10β-三羟基-6βH,7αH,11βH-1(5)-愈创木-12,6α-内酯；8α-当归酰氧基-1β,2β:4β,5β-二环氧-10β-羟基-6βH,7αH,11βH-12,6α-愈创木内酯；8α-当归酰氧基-4α,10β-二羟基-2-氧代-6βH,7αH,11βH-1(5)-愈创木-12,6α-内酯和8-去乙酰母菊内酯，并通过薄层色谱法、质谱法、红外光谱法、高效液相色谱法和二极管阵列检测对其进行了表征。纯化后的提取物通过快速色谱法进行分离。高效液相色谱分离采用不同的甲醇-水梯度作为流动相，以LiChrospher 100-RP8 5微米或Zorbax SB-C-色谱柱8 3.5微米作为固定相。将色谱数据与显示出抗炎作用的前薁类化合物8α-惕各酰氧基-阿尔塔辛的色谱数据进行比较。通过这些特性，也可以从其他来源鉴定具有潜在抗炎特性的愈创木内酯。