Sivagnaname N, Amalraj D D, Kalyanasundaram M, Das P K
Vector Control Research Centre (ICMR), Pondicherry, India.
Indian J Med Res. 2001 Jul;114:18-24.
BACKGROUND & OBJECTIVES: Review of literature indicates that none of the chemicals so far studied for oviposition attractancy was found to be promising for operational use. Efforts are being made to find an effective substance to corner a large number of ovipositing females to selective breeding sites for ease of control operation. This will reduce insecticidal consumption and will be cost-effective. This study is aimed to evaluate the oviposition attractancy of aqueous infusion from a wood inhabiting fungus (Polyporus spp.) for vector mosquitoes both in the laboratory and under field conditions.
An aqueous infusion (10%) was prepared by soaking 10 g of fungal powder in 100 ml of chlorine-free tap water. The mixture was shaken thoroughly and filtered through Whatman No. 1 filter paper. The filtered infusion was tested for oviposition attractancy against vector mosquitoes both in the laboratory and under field conditions.
The laboratory study on the dose-effect relationship indicated that at an optimum concentration of 4 ppm, maximum attractancy was observed. Water treated with aqueous fungal infusion (AFI) at 4 ppm received significantly more egg rafts/eggs of vector mosquitoes than other substrates like rearing water, natural breeding water and tap water. Trials conducted in the field showed that mud pots treated with AFI placed in both indoor and outdoor locations received significantly more Aedes aegypti eggs than the control. Similarly, treated pots placed in paddy fields attracted significantly more gravid Anopheles subpictus for oviposition than untreated pots. In contrast, the number of egg rafts of Culex quinquefasciatus laid in AFI treated pots was significantly less than in the control ones owing to strong natural olfactory factors associated with the breeding habitat.
INTERPRETATION & CONCLUSION: The results indicated that there is scope for developing a trap incorporating this oviposition attractant along with insect growth regulator(s) (IGR) for surveillance and/or control of Ae. aegypti and other vector mosquitoes breeding in paddy fields.
文献综述表明，迄今为止所研究的用于产卵引诱的化学物质，均未发现有用于实际操作的前景。目前正在努力寻找一种有效物质，以诱使大量产卵雌蚊前往选择性繁殖地点，便于进行控制操作。这将减少杀虫剂的使用量，并具有成本效益。本研究旨在评估一种木生真菌（多孔菌属）的水浸液对媒介蚊虫在实验室和野外条件下的产卵引诱力。
将10克真菌粉末浸泡在100毫升无氯自来水中，制备10%的水浸液。将混合物充分摇匀，并用1号沃特曼滤纸过滤。过滤后的浸液在实验室和野外条件下测试对媒介蚊虫的产卵引诱力。
实验室对剂量效应关系的研究表明，在最佳浓度4 ppm时，观察到最大引诱力。用4 ppm的真菌水浸液（AFI）处理的水比其他基质（如饲养用水、天然繁殖用水和自来水）接收的媒介蚊虫卵块/卵显著更多。在野外进行的试验表明，放置在室内和室外位置并用AFI处理的泥罐比对照接收的埃及伊蚊卵显著更多。同样，放置在稻田中的处理过的罐子比未处理的罐子吸引到显著更多的怀卵拟态按蚊产卵。相比之下，由于与繁殖栖息地相关的强烈自然嗅觉因素，在AFI处理过的罐子中产卵的致倦库蚊卵块数量显著少于对照。
结果表明，开发一种结合这种产卵引诱剂和昆虫生长调节剂（IGR）的诱捕器用于监测和/或控制埃及伊蚊及其他在稻田中繁殖的媒介蚊虫具有可行性。
