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完整人红细胞膜细胞质表面的光化学标记

Photochemical labeling of the cytoplasmic surface of the membranes of intact human erythrocytes.

作者信息

Staros J V, Richards F M

出版信息

J Biol Chem. 1975 Oct 25;250(20):8174-8.

PMID:1176465
Abstract

N-(4-azido-2-nitrophenyl)-2-aminoethyl sulfonate (NAP-taurine), a photolabile nitrene precursor, has been shown to permeate the human erythrocyte membrane at 37degrees but not at 0 degrees. Utilizing this differential permeability, we have loaded intact erythrocytes with NAP-[35S]taurine in the dark at 37degrees, cooled them to 0 degrees, washed them free of external NAP-[35S]taurine in the dark and cold, and photolyzed them, resulting in labeling of hemoglobin and of proteins on the cytoplasmic surface of the membrane. These experiments complement those previously reported on the labeling of the external surface of the membranes with this reagent (Staros, J. V., and Richards, F. M. (1974) Biochemistry 13, 2720-2726).

摘要

N-(4-叠氮基-2-硝基苯基)-2-氨基乙基磺酸盐（NAP-牛磺酸）是一种光不稳定的氮烯前体，已证明它在37℃时能透过人红细胞膜，但在0℃时则不能。利用这种差异通透性，我们在37℃黑暗条件下用NAP-[35S]牛磺酸加载完整红细胞，将其冷却至0℃，在黑暗和低温条件下洗涤以去除外部的NAP-[35S]牛磺酸，然后对其进行光解，从而使血红蛋白和膜细胞质表面的蛋白质被标记。这些实验补充了先前报道的用该试剂标记膜外表面的实验（Staros, J. V., and Richards, F. M. (1974) Biochemistry 13, 2720 - 2726）。

相似文献

1
Photochemical labeling of the cytoplasmic surface of the membranes of intact human erythrocytes.完整人红细胞膜细胞质表面的光化学标记
J Biol Chem. 1975 Oct 25;250(20):8174-8.
2
Topography of rhodopsin in rod outer segment disk membranes. Photochemical labeling with N-(4-azido-2-nitrophenyl)-2-aminoethanesulfonate.视杆细胞外段盘膜中视紫红质的拓扑结构。用N-(4-叠氮基-2-硝基苯基)-2-氨基乙烷磺酸盐进行光化学标记。
Biochemistry. 1980 Feb 19;19(4):684-91. doi: 10.1021/bi00545a012.
3
Transmembrane effects of irreversible inhibitors of anion transport in red blood cells. Evidence for mobile transport sites.红细胞中阴离子转运不可逆抑制剂的跨膜效应。移动转运位点的证据。
J Gen Physiol. 1979 Apr;73(4):493-514. doi: 10.1085/jgp.73.4.493.
4
Mechanism of anion exchange across the red cell membrane by band 3: interactions between stilbenedisulfonate and NAP-taurine binding sites.带3蛋白介导阴离子跨红细胞膜交换的机制：二苯乙烯二磺酸盐与NAP-牛磺酸结合位点之间的相互作用
Biochemistry. 1981 Sep 29;20(20):5695-701. doi: 10.1021/bi00523a009.
5
N-(4-azido-2-nitrophenyl)-2-aminoethylsulfonate (NAP-taurine) as a photoaffinity probe for identifying membrane components containing the modifier site of the human red blood cell anion exchange system.N-(4-叠氮基-2-硝基苯基)-2-氨基乙磺酸盐（NAP-牛磺酸）作为一种光亲和探针，用于鉴定含有人类红细胞阴离子交换系统修饰位点的膜成分。
J Gen Physiol. 1978 Nov;72(5):631-49. doi: 10.1085/jgp.72.5.631.
6
Photochemical labeling of the surface proteins of human erythrocytes.人红细胞表面蛋白的光化学标记
Biochemistry. 1974 Jun 18;13(13):2720-6. doi: 10.1021/bi00710a010.
7
Asymmetry of the red cell anion exchange system. Different mechanisms of reversible inhibition by N-(4-azido-2-nitrophenyl)-2-aminoethylsulfonate (NAP-taurine) at the inside and outside of the membrane.红细胞阴离子交换系统的不对称性。膜内外N-(4-叠氮基-2-硝基苯基)-2-氨基乙磺酸盐（NAP-牛磺酸）可逆抑制的不同机制。
J Gen Physiol. 1978 Nov;72(5):607-30. doi: 10.1085/jgp.72.5.607.
8
Interactions of NIP-taurine, NAP-taurine, and Cl- with the human erythrocyte anion exchange system.NIP-牛磺酸、NAP-牛磺酸及氯离子与人类红细胞阴离子交换系统的相互作用。
Am J Physiol. 1987 Nov;253(5 Pt 1):C652-61. doi: 10.1152/ajpcell.1987.253.5.C652.
9
Use of NAP-taurine as a photoaffinity probe for the human erythrocyte anion exchange system.使用NAP-牛磺酸作为人类红细胞阴离子交换系统的光亲和探针。
Ann N Y Acad Sci. 1980;346:212-31. doi: 10.1111/j.1749-6632.1980.tb22101.x.
10
NIP- and NAP-taurine bind to external modifier site of AE1 (band 3), at which iodide inhibits anion exchange.NIP-和NAP-牛磺酸与AE1（带3）的外部修饰位点结合，碘化物在该位点抑制阴离子交换。
Am J Physiol. 1995 Aug;269(2 Pt 1):C410-6. doi: 10.1152/ajpcell.1995.269.2.C410.

引用本文的文献

1
Lactoperoxidase-125I localization of salt-extractable alkaline phosphatase on the cytoplasmic membrane of Bacillus licheniformis.乳过氧化物酶-125I对嗜热栖热放线菌细胞质膜上可盐提取碱性磷酸酶的定位 。 （注：原文中“Bacillus licheniformis”有误，根据语境推测应为“Thermus thermophilus”，这里按照推测后的物种名翻译，若有错误请指出）
J Bacteriol. 1981 Feb;145(2):934-45. doi: 10.1128/jb.145.2.934-945.1981.
2
Proteins of the kidney microvillar membrane. Asymmetric labelling of the membrane by lactoperoxidase-catalysed radioiodination and by photolysis of 3,5-di[125I]iodo-4-azidobenzenesulphonate.肾微绒毛膜蛋白。通过乳过氧化物酶催化的放射性碘化以及3,5-二[125I]碘-4-叠氮基苯磺酸盐的光解对膜进行不对称标记。
Biochem J. 1980 Apr 1;187(1):31-44. doi: 10.1042/bj1870031.
3
Asymmetry of the red cell anion exchange system. Different mechanisms of reversible inhibition by N-(4-azido-2-nitrophenyl)-2-aminoethylsulfonate (NAP-taurine) at the inside and outside of the membrane.
红细胞阴离子交换系统的不对称性。膜内外N-(4-叠氮基-2-硝基苯基)-2-氨基乙磺酸盐（NAP-牛磺酸）可逆抑制的不同机制。
J Gen Physiol. 1978 Nov;72(5):607-30. doi: 10.1085/jgp.72.5.607.
4
Transmembrane effects of irreversible inhibitors of anion transport in red blood cells. Evidence for mobile transport sites.红细胞中阴离子转运不可逆抑制剂的跨膜效应。移动转运位点的证据。
J Gen Physiol. 1979 Apr;73(4):493-514. doi: 10.1085/jgp.73.4.493.
5
Asymmetric distribution of plasma membrane proteins in mouse L-929 cells.小鼠L-929细胞中质膜蛋白的不对称分布。
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6235-9. doi: 10.1073/pnas.76.12.6235.
6
Labeling of complex III, with [35S]diazobenzenesulfonate: orientation of this electron transfer segment in the mitochondrial inner membrane.用[35S]重氮苯磺酸盐对细胞色素bc1复合体进行标记：该电子传递片段在线粒体内膜中的取向
Proc Natl Acad Sci U S A. 1979 Feb;76(2):741-5. doi: 10.1073/pnas.76.2.741.