Emptage N J
Department of Pharmacology, University of Oxford, UK.
Curr Opin Pharmacol. 2001 Oct;1(5):521-5. doi: 10.1016/s1471-4892(01)00090-x.
The use of fluorescent imaging techniques in the study of living biological systems has become an important experimental tool in modern biology. Over recent years novel imaging technologies have been developed and older techniques refined. New fluorescent probes continue to become available and the ways in which they are used is increasingly creative. Commonly used imaging methods such as confocal and multiphoton microscopy, when combined with techniques such as fluorescence resonance energy transfer (FRET) and fluorescence lifetime imaging (FLIM), can provide powerful strategies with which to study molecular events in intact cells.
荧光成像技术在活生物系统研究中的应用已成为现代生物学中一种重要的实验工具。近年来，新型成像技术不断涌现，旧技术也得以改进。新型荧光探针不断问世，其使用方式也越来越富有创造性。常用的成像方法，如共聚焦显微镜和多光子显微镜，与荧光共振能量转移（FRET）和荧光寿命成像（FLIM）等技术相结合，可为研究完整细胞中的分子事件提供强大的策略。
