Zhan H, Chen L M, Xin Y M, Tang G X, Wen J
Institute of Aviation Medicine, Beijing, China.
Space Med Med Eng (Beijing). 1999 Feb;12(1):1-5.
To observe the effects of repeated +10 Gz stress on cerebral lipid peroxidation, liver and renal function in rats and the prophylactic effects of antioxidant tea polyphenols (TP).
Twenty-four male Wistar rats were randomly divided into three groups (n = 8, each): group A (control), group B (+10 Gz), and group C (TP). Group B and C were exposed to repeated +10 Gz stress (each for 30s, onset rate about 0.5 G/s, 3 times/d with +1 Gz 1 min intervals, 3 d/wk, 4 weeks in total), but group A was only submitted to +1 Gz. TP (200 mg/kg) was given orally in group C about 1 h prior to the +Gz experiment, while distilled water was given in group A and B. Lipid peroxidation in the brain, liver and renal functions and serum lipids were determined.
As compared with the control, lipid peroxidation in rat cerebral homogenate, mitochondria and cytoplasm was significantly increased (P < 0. 05), and serum creatinine concentration was markedly elevated after repeated +10 Gz stress (P < 0. 01). But, TP had significant inhibitory effect on +10 Gz stress induced peroxidative injury in rat brain and reduced the serum creatinine level. There were no differences of serum triglyceride and total cholesterol concentrations and glutamic-pyruvic transaminase activity among the three groups.
These results indicated that repeated high +Gz stress could bring about peroxidative injury in brain and harmful effect on renal function, and natural antioxidant TP had significant protective effects.
观察重复 +10 Gz 应激对大鼠脑脂质过氧化、肝肾功能的影响以及抗氧化剂茶多酚（TP）的预防作用。
将 24 只雄性 Wistar 大鼠随机分为三组（每组 n = 8）：A 组（对照组）、B 组（+10 Gz 组）和 C 组（TP 组）。B 组和 C 组接受重复 +10 Gz 应激（每次 30 秒，起始速率约 0.5 G/s，每天 3 次，每次间隔 +1 Gz 1 分钟，每周 3 天，共 4 周），但 A 组仅接受 +1 Gz。C 组在 +Gz 实验前约 1 小时口服 TP（200 mg/kg），而 A 组和 B 组给予蒸馏水。测定脑脂质过氧化、肝肾功能和血脂。
与对照组相比，重复 +10 Gz 应激后大鼠脑匀浆、线粒体和细胞质中的脂质过氧化显著增加（P < 0.05），血清肌酐浓度显著升高（P < 0.01）。但是，TP 对 +10 Gz 应激诱导的大鼠脑过氧化损伤具有显著抑制作用，并降低了血清肌酐水平。三组之间血清甘油三酯、总胆固醇浓度和谷丙转氨酶活性无差异。
这些结果表明，重复的高 +Gz 应激可导致脑过氧化损伤和对肾功能产生有害影响，天然抗氧化剂 TP 具有显著的保护作用。
