Washizu T, Takahashi M, Azakami D, Ikeda M, Arai T
Department of Veterinary Science, Nippon Veterinary and Animal Science University, Musashino, Tokyo, Japan.
Vet Res Commun. 2001 Dec;25(8):623-9. doi: 10.1023/a:1012787012433.
The activities of the enzymes involved in the malate-aspartate shuttle and the expression of malate dehydrogenase (MDH), a rate-limiting enzyme in the NADH shuttle that produces ATP in glucose metabolism in leukocytes, were determined to investigate the differences in this shuttle system in the peripheral leukocytes of dogs and cats. There were no significant differences between dogs and cats in plasma glucose, immunoreactive insulin, free fatty acid or triglyceride concentrations. The activities of cytosolic and mitochondrial MDH and of mitochondrial glutamate dehydrogenase (GLDH) in canine leukocytes were significantly higher than in feline leukocytes. High activities of MDH in canine leukocytes were confirmed by RT-PCR analysis on the total RNA extracted from leukocytes. It was concluded that there were significant differences between dogs and cats in the NADH shuttle system.
为了研究犬和猫外周血白细胞中苹果酸 - 天冬氨酸穿梭系统的差异，测定了参与苹果酸 - 天冬氨酸穿梭的酶的活性以及苹果酸脱氢酶（MDH）的表达，MDH是白细胞葡萄糖代谢中产生ATP的NADH穿梭的限速酶。犬和猫在血浆葡萄糖、免疫反应性胰岛素、游离脂肪酸或甘油三酯浓度方面没有显著差异。犬白细胞中胞质和线粒体MDH以及线粒体谷氨酸脱氢酶（GLDH）的活性显著高于猫白细胞。通过对从白细胞中提取的总RNA进行RT-PCR分析，证实了犬白细胞中MDH的高活性。得出的结论是，犬和猫在NADH穿梭系统中存在显著差异。
