日本石垣岛犬蜱虫感染及蜱传埃立克体感染调查

Survey of tick infestation and tick-borne ehrlichial infection of dogs in Ishigaki Island, Japan.

作者信息

Inokuma H, Nane G, Uechi T, Yonahara Y, Brouqui P, Okuda M, Onishi T

机构信息

Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, Yoshida, Japan.

出版信息

J Vet Med Sci. 2001 Nov;63(11):1225-7. doi: 10.1292/jvms.63.1225.

DOI:10.1292/jvms.63.1225

PMID:11767058

Abstract

Twelve (54.5%) of 22 free-roaming dogs in Ishigaki Island had tick infestation identified as Rhipicephalus sanguineus. There were 121 ticks recovered and consisted of 28 females, 58 males, 22 nymphs and 3 larvae. Infection of dogs possibly with canine ehrlichial pathogens was examined by both indirect immunofluorescence assay and polymerase chain reaction (PCR). Two dogs of the 13 examined were sero-positive for the human granulocytic ehrlichia agent, and one of two dogs was PCR positive for Ehrlichia platys. This dog had platelet numbers slightly lower than normal value, however, no morulae were found within platelet on peripheral blood smear stained with Giemsa.

摘要

石垣岛22只自由放养犬中有12只（54.5%）被鉴定感染血红扇头蜱。共采集到121只蜱，其中雌蜱28只、雄蜱58只、若蜱22只、幼虫3只。通过间接免疫荧光试验和聚合酶链反应（PCR）检测犬只可能感染犬埃立克体病原体的情况。在接受检查的13只犬中，有2只对人粒细胞埃立克体呈血清学阳性，在这2只犬中有1只对血小板埃立克体PCR检测呈阳性。这只犬的血小板数量略低于正常值，然而，在经吉姆萨染色的外周血涂片上，血小板内未发现桑葚体。