Rivory L P, Clarke S J, Boyer M, Bishop J F
Sydney Cancer Centre, Royal Prince Alfred Hospital, Camperdown, NSW, Australia.
J Chromatogr B Biomed Sci Appl. 2001 Dec 25;765(2):135-40. doi: 10.1016/s0378-4347(01)00406-6.
A reversed-phase high-performance liquid chromatography method was developed and validated for the quantitation of pemetrexed (LY231514, ALIMTA) in human urine and plasma. Plasma samples were spiked with the internal standard lometrexol and extracted using Certify II columns. Pemetrexed was assayed in diluted urine by an external calibration method. A C8 column was used for the separation of analytes with a mobile phase composed of sodium formate buffer and acetonitrile. Between- and within-day precision and accuracy were acceptable down to the limit of quantitation of 5 ng/ml in plasma. This method was used successfully for an investigation of the disposition of pemetrexed in patients receiving 500 mg/m2 as a 10-min infusion.
建立了一种反相高效液相色谱法并进行了验证，用于定量测定人尿液和血浆中的培美曲塞（LY231514，力比泰）。血浆样品加入内标洛美曲唑后，使用Certify II柱进行萃取。培美曲塞在稀释尿液中采用外标法进行测定。使用C8柱，以甲酸钠缓冲液和乙腈组成的流动相分离分析物。血浆中低至5 ng/ml的定量限的日间和日内精密度及准确度均可接受。该方法成功用于研究接受500 mg/m² 10分钟静脉滴注的患者体内培美曲塞的处置情况。
