Terao J, Miyoshi M, Miyamoto S
Department of Nutrition, School of Medicine, The University of Tokushima, Japan.
J Chromatogr B Biomed Sci Appl. 2001 Dec 25;765(2):199-203. doi: 10.1016/s0378-4347(01)00426-1.
A blotting technique was developed to specifically detect lipid hydroperoxides in thin-layer chromatography. Phosphatidylcholine hydroperoxides and cholesteryl linoleate hydroperoxides ranging from 0.1 to 0.5 nmol, which were prepared by reaction with soybean lipoxygenase, were visualized as fluorescent spots on the blotted membrane by immersing the plate into a blotting solvent containing 0.01% (w/v) diphenyl-1-pyrenylphosphine. This technique was applied successfully to monitor lipid peroxidation in human low-density lipoprotein in vitro.
开发了一种印迹技术，用于在薄层色谱中特异性检测脂质氢过氧化物。通过与大豆脂氧合酶反应制备的0.1至0.5 nmol的磷脂酰胆碱氢过氧化物和亚油酸胆固醇酯氢过氧化物，通过将板浸入含有0.01%（w/v）二苯基-1-芘基膦的印迹溶剂中，在印迹膜上显示为荧光斑点。该技术已成功应用于体外监测人低密度脂蛋白中的脂质过氧化。
