Scornik J C, Drewinko B
J Immunol. 1975 Nov;115(5):1223-6.
Human lymphoid spleen cells attached to Petri dishes by poly-L-lysine bind 51Cr-labeled erythrocytes coated with IgG antibodies or complement but not uncoated erythrocytes or those coated with IgM antibodies. The number of erythrocytes bound through complement receptors is several times larger than that bound through IgG receptors. Increasing up to five times the number of IgG molecules on the red blood cells only leads to a slight increase of binding. However, the addition of complement to the IgG-coated erthrocytes increases 10 times the binding to spleen cells, even in the presence of an excess of normal IgG. These results can be explained by postulating that there is a larger number (or greater affinity) of spleen cell receptors for complement than that of spleen cell receptors for IgG.
通过聚-L-赖氨酸附着在培养皿上的人淋巴样脾细胞能结合包被有IgG抗体或补体的51Cr标记红细胞，但不能结合未包被的红细胞或包被有IgM抗体的红细胞。通过补体受体结合的红细胞数量比通过IgG受体结合的红细胞数量大几倍。红细胞上IgG分子数量增加至五倍仅导致结合略有增加。然而，即使存在过量的正常IgG，向包被有IgG的红细胞中添加补体也会使与脾细胞的结合增加10倍。这些结果可以通过假设脾细胞对补体的受体数量（或亲和力）比对IgG的受体数量更大来解释。
